Higham S M, Edgar W M
Department of Dental Sciences, School of Dental Surgery, Liverpool, England.
Arch Oral Biol. 1989;34(5):329-34. doi: 10.1016/0003-9969(89)90105-2.
The relationship between these factors was studied in plaque and plaque fluid samples taken at intervals during the Stephan pH curve following a sucrose mouth rinse. Levels of lactate rose after the rinse, then fell during the pH recovery phase. Levels of acetate, propionate and phosphate fell after rinsing, then rose again. Amino acid concentrations also changed, with many showing a fall followed by a rise; others rising then falling; and some showing a more variable or complex pattern. In resting plaque fluid, only alanine, proline, glutamic acid, glycine and ammonia were present at concentrations above 1 mmol/l. Delta-aminovaleric acid was detected at levels below those that have been found in monkeys. Hydroxyproline and hydroxylysine were consistently detected, levels of arginine were generally low, and those of cystine consistently very low. The results may provide a basis for understanding the complex metabolic interrelations that occur in the course of the Stephan curve and which may reflect or produce the observed pH changes. They suggest that besides the amount of acid produced, the type of acid, buffering power and base production should be considered as determinants of plaque pH.
在蔗糖漱口后Stephan pH曲线期间，间隔采集菌斑和菌斑液样本，研究了这些因素之间的关系。漱口后乳酸水平升高，然后在pH恢复阶段下降。醋酸盐、丙酸盐和磷酸盐水平在漱口后下降，然后再次上升。氨基酸浓度也发生了变化，许多呈现先下降后上升；其他的则先上升后下降；还有一些呈现出更具变化性或复杂性的模式。在静止的菌斑液中，只有丙氨酸、脯氨酸、谷氨酸、甘氨酸和氨的浓度高于1 mmol/l。检测到的δ-氨基戊酸水平低于在猴子中发现的水平。始终检测到羟脯氨酸和羟赖氨酸，精氨酸水平通常较低，胱氨酸水平始终非常低。这些结果可能为理解在Stephan曲线过程中发生的复杂代谢相互关系提供基础，这些相互关系可能反映或产生观察到的pH变化。结果表明，除了产生的酸量外，酸的类型、缓冲能力和碱的产生也应被视为菌斑pH的决定因素。
