Gurevich A I, Esipov R S, Kachalina T A, Kaliushin A L, Korosteleva M D
Bioorg Khim. 1995 Feb;21(2):117-23.
The comparison of expression levels of two genes-interleukin-3 (il3) and epidermal growth factor connected to leader peptide of OmpF (lompegf)-was carried out using specially constructed plasmids contained various structures of translational enhancers. It was shown that besides already known binding sites from mRNA translation initiation region (TIR) to 16S rRNA (SD, UB1 and DB), there is additional binding site of TIR disposed from -30 to -60 nt upstream of start codon AUG (UB2) having significant increasing effect on translation initiation and correspondingly on expression level.
利用包含各种翻译增强子结构的特制质粒，对两个基因——白细胞介素-3（il3）和与外膜蛋白F前导肽相连的表皮生长因子（lompegf）的表达水平进行了比较。结果表明，除了已知的从mRNA翻译起始区域（TIR）到16S rRNA的结合位点（SD、UB1和DB）外，在起始密码子AUG上游-30至-60 nt处还存在TIR的额外结合位点（UB2），该位点对翻译起始以及相应的表达水平具有显著的增强作用。
