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[大肠杆菌中基因表达水平对翻译起始区段（TIR）结构的依赖性]

[Dependence of the level of gene expression in E. coli on the structure of the translation initiation segment (TIR)].

作者信息

Gurevich A I, Esipov R S, Kachalina T A, Kaiushin A L

出版信息

Bioorg Khim. 1995 Apr;21(4):282-8.

PMID:7786319
Abstract

The expression levels of genes that are transcribed to give mRNAs with identical leader sequences and even with identical extended coding regions may differ considerably. In order to determine the mechanism of this phenomenon, secondary structures of some mRNAs synthesized from a series of expression plasmids were studied. It was shown that the effect of the mRNA secondary structure in the translation initiation region on the initiation efficiency is due not only to the hairpin formation in this region but also to long-range interactions. When complementary structures tighter than those resulted from the interaction of regions SD, UB1, UB2, and DB with 16S rRNA are formed, the efficiency of the translation initiation and, consequently, the expression level decrease.

摘要

转录产生具有相同前导序列甚至相同延伸编码区的mRNA的基因，其表达水平可能有很大差异。为了确定这种现象的机制，研究了从一系列表达质粒合成的一些mRNA的二级结构。结果表明，mRNA二级结构在翻译起始区域对起始效率的影响不仅归因于该区域的发夹形成，还归因于长程相互作用。当形成比SD、UB1、UB2和DB区域与16S rRNA相互作用产生的互补结构更紧密的结构时，翻译起始效率以及因此的表达水平会降低。

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Bioorg Khim. 1995 Apr;21(4):282-8.
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