Frau M, Colombo G, Marchese G, Stefanini E, Gessa G L
Bernard B. Brodie Department of Neuroscience, University of Cagliari, Italy.
Alcohol Alcohol. 1995 Jan;30(1):133-7.
Specific gamma-hydroxybutyric acid (GHB) binding sites in cortical membranes of selectively bred alcohol-preferring sP and alcohol-non preferring sNP rats were compared using [2,3(-3)H]GHB ligand. The sP rat line showed an increased affinity (approximately 40% lower Kd) of both the high- and low-affinity sites in comparison with the sNP line. No significant difference in GHB receptor density (Bmax) was detected between the two rat lines. The results raise the possibility that differences in GHB binding sites may play a role in the genetic predisposition to ethanol preference in our rat line.
使用[2,3(-3)H]γ-羟基丁酸（GHB）配体，比较了选择性培育的嗜酒sP大鼠和不嗜酒sNP大鼠皮质膜中特定的GHB结合位点。与sNP品系相比，sP大鼠品系的高亲和力和低亲和力位点的亲和力均有所增加（解离常数Kd约低40%）。在两个大鼠品系之间未检测到GHB受体密度（最大结合容量Bmax）的显著差异。这些结果增加了一种可能性，即GHB结合位点的差异可能在我们的大鼠品系对乙醇偏好的遗传易感性中起作用。
