Adickes E D, Mollner T J, Makoid M C
Department of Pathology, Creighton University School of Medicine, Omaha, NE 68178, USA.
Alcohol Alcohol Suppl. 1993;2:283-8.
The development of an in vitro cardiogenesis model was designed to enhance our understanding of the mechanism(s) of ethanol teratogenicity. Growth and development events in the model are similar to in vivo events. Time-specific and event-specific windows of biokinetic activities during both hyperplastic and hypertrophic growth are easily controlled and independently investigated. Systematic study of the effects of ethanol on the embryogenesis model, from committed blast cells to mature, functioning cells was accomplished to determine at what stage or stages of the growth and development paradigm ethanol exerts its teratogenic potential. Using the model and comparing the data to in vivo ethanol exposures, it appears that ethanol impairs the capability of the cell to properly propagate and mature.
体外心脏发生模型的开发旨在增进我们对乙醇致畸机制的理解。该模型中的生长和发育事件与体内事件相似。在增生性和肥厚性生长过程中，生物动力学活动的时间特异性和事件特异性窗口易于控制且可独立研究。对乙醇从定向胚细胞到成熟功能细胞的胚胎发生模型的影响进行了系统研究，以确定乙醇在生长和发育模式的哪个阶段发挥其致畸潜力。使用该模型并将数据与体内乙醇暴露情况进行比较，结果显示乙醇会损害细胞正常增殖和成熟的能力。
