Bahrenscheer J, Kemp M, Kurtzhals J A, Gachihi G S, Kharazmi A, Theander T G
Centre for Medical Parasitology, University of Copenhagen, Denmark.
APMIS. 1995 Feb;103(2):131-9.
Crude preparations of Leishmania donovani proteins were separated by preparative SDS-polyacrylamide gel electrophoresis. Fractions of separated proteins were recovered by electroelution directly from the gel into separate chambers. The isolated protein fractions were tested for induction of proliferation and interferon-gamma (IFN-gamma) production in cultures of peripheral blood mononuclear cells (PBMC) from individuals who had recovered from visceral leishmaniasis caused by L. donovani. The release of interleukin-4 (IL-4) by PBMC stimulated with the isolated L. donovani antigen fractions was measured after treatment with phorbol-myristate-acetate and ionomycin. The cells proliferated in response to all protein fractions with molecular weights in the range < 12 kDa to 85 kDa. In general, IFN-gamma was secreted in response to stimulation with all the protein fractions, whereas IL-4 production was infrequently observed. The results show that T cells from individuals who have been cured of visceral leishmaniasis recognize and respond to a wide range of leishmanial antigens. There was no evidence of particular fractions constantly giving either IFN-gamma or IL-4-producing responses.
杜氏利什曼原虫蛋白质的粗制品通过制备性十二烷基硫酸钠-聚丙烯酰胺凝胶电泳进行分离。分离出的蛋白质组分通过电洗脱直接从凝胶中回收至单独的腔室。对从由杜氏利什曼原虫引起的内脏利什曼病中康复的个体的外周血单核细胞（PBMC）培养物中，检测分离出的蛋白质组分诱导增殖和干扰素-γ（IFN-γ）产生的能力。在用佛波醇-肉豆蔻酸酯-乙酸酯和离子霉素处理后，测量用分离出的杜氏利什曼原虫抗原组分刺激的PBMC释放白细胞介素-4（IL-4）的情况。细胞对分子量范围在<12 kDa至85 kDa的所有蛋白质组分均有增殖反应。一般来说，所有蛋白质组分刺激后均分泌IFN-γ，而很少观察到IL-4的产生。结果表明，已治愈内脏利什曼病的个体的T细胞能够识别并对多种利什曼原虫抗原作出反应。没有证据表明存在特定的组分始终引发产生IFN-γ或IL-4的反应。
