Lind M, Deleuran B, Thestrup-Pedersen K, Søballe K, Eriksen E F, Bünger C
Department of Orthopedic Surgery, University Hospital of Aarhus, Denmark.
APMIS. 1995 Feb;103(2):140-6.
The in vitro chemotactic response of human osteoblasts was investigated towards the following growth factors: TGF-beta, PDGFs, FGFs and IGFs. Human osteoblasts grown from trabecular bone after enzymatic digestion were studied. TGF-beta stimulated the migration of human osteoblasts in a dose-dependent manner with a four-fold increase in migrated cells at 100 pg/ml, which was the optimum concentration. PDGF-BB also stimulated migration four-fold in a dose-dependent manner with a maximum response at 10 ng/ml. PDGF-AA, IGF-I and IGF-II stimulated migration two-fold at 100 ng/ml. The results show that TGF-beta and PDGF-BB are important regulators of human osteoblast migration, but other growth factors IGF-I, IGF-II and PDGF-AA may also stimulate osteoblast migration. Our results additionally suggest that TGF-beta and PDGF-BB may participate in the recruitment of osteoblasts during bone remodeling since both TGF-beta and PDGF-BB are found in bone matrix and could be released during osteoclastic bone resorption. They furthermore support a possible use of TGF-beta and PDGF-BB in growth factor-induced osteogenesis.
转化生长因子-β（TGF-β）、血小板衍生生长因子（PDGFs）、成纤维细胞生长因子（FGFs）和胰岛素样生长因子（IGFs）。对经酶消化后从松质骨培养的人成骨细胞进行了研究。TGF-β以剂量依赖的方式刺激人成骨细胞迁移，在100 pg/ml时迁移细胞增加了四倍，这是最佳浓度。血小板衍生生长因子-BB（PDGF-BB）也以剂量依赖的方式使迁移增加四倍，在10 ng/ml时达到最大反应。血小板衍生生长因子-AA（PDGF-AA）、胰岛素样生长因子-I（IGF-I）和胰岛素样生长因子-II（IGF-II）在100 ng/ml时使迁移增加两倍。结果表明，TGF-β和PDGF-BB是人成骨细胞迁移的重要调节因子，但其他生长因子IGF-I、IGF-II和PDGF-AA也可能刺激成骨细胞迁移。我们的结果还表明，TGF-β和PDGF-BB可能参与骨重塑过程中成骨细胞的募集，因为在骨基质中都发现了TGF-β和PDGF-BB，并且它们可能在破骨细胞骨吸收过程中释放。这些结果进一步支持了TGF-β和PDGF-BB在生长因子诱导的骨生成中的可能应用。
