Celotti Fabio, Colciago A, Negri-Cesi P, Pravettoni A, Zaninetti R, Sacchi M C
Institute of Endocrinology, University of Milano, Milano, Italy.
Wound Repair Regen. 2006 Mar-Apr;14(2):195-202. doi: 10.1111/j.1743-6109.2006.00110.x.
Platelet-enriched plasma (PRP) is used in therapy as a source of growth factors in bone fracture and wound healing; however, few data exist on its role in the different aspects of the healing process. The effect of PRP and of the two main growth factors present in this preparation (platelet-derived growth factor [PDGF] and transforming growth factor-beta [TGF-beta]) was evaluated in vitro using the human osteoblastic cell line SaOS-2, which was shown by reverse transcription-polymerase chain reaction to express both PDGF-alpha and -beta receptors. Batroxobine-activated PRP was added in different concentrations to SaOS-2 cells to assess cell migration (by a microchemotaxis assay) and cell proliferation (by [3H]-thymidine incorporation into the DNA). Immunoneutralization with anti-PDGF-beta or anti-TGF-beta antibodies allowed the assessment of the specific role of these growth factors. The overall results obtained indicate that PRP dose-dependently stimulates both chemotaxis and cell proliferation. PDGF and TGF-beta appear to exert distinct effects on the two parameters, the former involved in stimulating cell migration and the latter in inhibiting cell proliferation. It is concluded that the different growth factors present in activated PRP can specifically contribute to the main processes of tissue regeneration.
富含血小板血浆（PRP）作为骨折和伤口愈合中生长因子的来源用于治疗；然而，关于其在愈合过程不同方面作用的数据很少。使用人成骨细胞系SaOS-2在体外评估了PRP以及该制剂中存在的两种主要生长因子（血小板衍生生长因子[PDGF]和转化生长因子-β[TGF-β]）的作用，逆转录-聚合酶链反应显示该细胞系表达PDGF-α和-β受体。将巴曲酶激活的PRP以不同浓度添加到SaOS-2细胞中，以评估细胞迁移（通过微趋化性测定）和细胞增殖（通过[3H]胸腺嘧啶掺入DNA）。用抗PDGF-β或抗TGF-β抗体进行免疫中和可评估这些生长因子的特定作用。获得的总体结果表明，PRP剂量依赖性地刺激趋化性和细胞增殖。PDGF和TGF-β似乎对这两个参数发挥不同作用，前者参与刺激细胞迁移，后者参与抑制细胞增殖。得出的结论是，活化PRP中存在的不同生长因子可特异性促进组织再生的主要过程。
