Mylvaganam H, Gilja O H, Halstensen A, Høiby E A, Digranes A, Bjorvatn B
Centre for International Health, University of Bergen, Norway.
APMIS. 1995 Feb;103(2):147-53.
Following an outbreak involving 3 cases of serogroup B meningococcal disease in a rural part of Western Norway, 2 clinical and 99 carrier isolates of Neisseria meningitidis were examined by small-fragment restriction endonuclease analysis (SF-REA) using EcoRI, to determine its potential for strain differentiation. SF-REA characterized all isolates and provided reproducible results with acceptable inter-clonal differentiation. The results of SF-REA correlated well with those of serological typing and were used to determine clonal diversity and prevalence of invasive strains among the carrier isolates. SF-REA was also useful in demonstrating acquisition of a new carrier strain after eradication of the initial strain by ofloxacin. Thirty-one different restriction patterns/pattern complexes were recognized among the 101 isolates. The two clinical isolates had identical restriction patterns and showed > or = 90% similarity to those of six carrier isolates. In three out of six apparent treatment failures, successful eradication of the original strain by ofloxacin was demonstrated by SF-REA. SF-REA proved valuable in strain differentiation of Neisseria meningitidis, complemented serology, and characterized all isolates which could not be typified by serology.
在挪威西部农村地区发生了一起涉及3例B群脑膜炎球菌病的疫情后，使用EcoRI通过小片段限制性内切酶分析（SF-REA）对2株临床分离株和99株脑膜炎奈瑟菌携带者分离株进行了检测，以确定其菌株分化潜力。SF-REA对所有分离株进行了分型，并提供了具有可接受的克隆间分化的可重复结果。SF-REA的结果与血清学分型结果相关性良好，用于确定携带者分离株中侵袭性菌株的克隆多样性和流行情况。SF-REA还可用于证明在使用氧氟沙星根除初始菌株后获得了一种新的携带者菌株。在101株分离株中识别出31种不同的限制性图谱/图谱复合体。这两株临床分离株具有相同的限制性图谱，并且与六株携带者分离株的图谱显示出≥90%的相似性。在六例明显的治疗失败案例中，有三例通过SF-REA证明了氧氟沙星成功根除了原始菌株。SF-REA在脑膜炎奈瑟菌的菌株分化中被证明是有价值的，补充了血清学，并对所有无法通过血清学分型的分离株进行了分型。
