Belmadani A, Combourieu I, Bonini M, Creppy E E
Laboratoire de Toxicologie, Université Bordeaux II, France.
Hum Exp Toxicol. 1995 Jan;14(1):34-7. doi: 10.1177/096032719501400108.
Fluvoxamine is an antidepressant drug introduced into the clinic in 1986. It acts by selectively inhibiting neuronal serotonin recapture. It can be quantified by several methods, including high performance liquid chromatography. The HPLC method used so far needs special equipment and has poor sensitivity. The technique is difficult and time consuming. An easier, quicker and more sensitive HPLC assay for the routine determination of fluvoxamine in human plasma has therefore been developed. After alkalinisation and direct extraction by a mixture of n-hexane-isoamylic alcohol 985: 15 (v/v) of plasma samples, the organic phases were further extracted by HCl 0.1 N. Thirty microL of the final extract (with loxapine as internal standard) were injected directly into a C-8 column with a mobile phase consisting of 370 mL acetonitrile, 0.4 mL diethylamine, 630 mL of distilled water, 25 mL pic B5. UV detection at 254 nm was used. The whole process was completed in 40 min. The detection limit was 10 ng mL-1. No interference was found either with several benzodiazepines or with antidepressant drugs commonly associated during treatments.
氟伏沙明是一种于1986年引入临床的抗抑郁药物。它通过选择性抑制神经元5-羟色胺重摄取发挥作用。它可以通过多种方法进行定量，包括高效液相色谱法。迄今为止使用的高效液相色谱法需要特殊设备且灵敏度较差。该技术难度大且耗时。因此，已开发出一种更简便、快速且灵敏的高效液相色谱法，用于常规测定人血浆中的氟伏沙明。将血浆样品用正己烷 - 异戊醇985:15（v/v）的混合物进行碱化和直接萃取后，有机相再用0.1N盐酸进一步萃取。将30微升最终萃取液（以洛沙平作为内标）直接注入C - 8柱，流动相由370毫升乙腈、0.4毫升二乙胺、630毫升蒸馏水、25毫升pic B5组成。采用254纳米处的紫外检测。整个过程在40分钟内完成。检测限为10纳克/毫升。在几种苯二氮䓬类药物或治疗期间通常联用的抗抑郁药物中均未发现干扰。
