Wong S H, Kranzler H R, DellaFera S, Fernandes R
Department of Laboratory Medicine, University of Connecticut School of Medicine, Farmington.
Biomed Chromatogr. 1994 Nov-Dec;8(6):278-82. doi: 10.1002/bmc.1130080605.
Fluvoxamine, a serotonin re-uptake inhibitor, was quantified in plasma by modifying a previously published procedure for monitoring plasma concentrations of tricyclic antidepressants. Alkalinized plasma samples were extracted with n-hexane/isoamyl alcohol, followed by back-extraction with diluted phosphoric acid. The extracts were analysed by reversed-phase liquid chromatography using a C-18 column, with phosphate/acetonitrile as the mobile phase. The assay was linear from 10 to 800 micrograms/L. Precision studies showed within-run and day-to-day coefficients of variation to be 4.5 and 6.8%, respectively. Desipramine interfered with the detection of fluvoxamine. The assay was used to measure a total of 8 plasma samples from 4 alcohol-dependent patients medicated with fluvoxamine as an adjunct to relapse prevention psychotherapy. In these patients, the plasma concentrations ranged from 54 to 241 micrograms/L. Dosage of fluvoxamine, duration of treatment, interval between last dosage and blood collection were associated with effects on plasma concentrations that were consistent with the pharmacokinetic profile of fluvoxamine.
氟伏沙明是一种血清素再摄取抑制剂，通过修改先前发表的用于监测三环类抗抑郁药血浆浓度的方法来对血浆中的氟伏沙明进行定量。将碱化的血浆样品用正己烷/异戊醇萃取，然后用稀磷酸进行反萃取。萃取物采用反相液相色谱法，使用C-18柱，以磷酸盐/乙腈作为流动相进行分析。该测定法在10至800微克/升范围内呈线性。精密度研究表明批内和批间变异系数分别为4.5%和6.8%。地昔帕明干扰氟伏沙明的检测。该测定法用于测量4名酒精依赖患者服用氟伏沙明作为预防复发心理治疗辅助药物后的总共8份血浆样品。在这些患者中，血浆浓度范围为54至241微克/升。氟伏沙明的剂量、治疗持续时间、最后一剂与采血之间的间隔与对血浆浓度的影响相关，这与氟伏沙明的药代动力学特征一致。
