Molecular cloning of Xenopus HGF cDNA and its expression studies in Xenopus early embryogenesis.

We isolated Xenopus HGF cDNA and examined its expression pattern in Xenopus early embryos and their dissected parts. Xenopus HGF consists of 710 amino acids and contains four kringle domains and serine protease-like structure just like mammalian HGF. Northern blot analysis showed that expression of Xenopus HGF mRNA starts at the late gastrula stage and its level increases during the period of later embryogenesis. Dissection experiments revealed that Xenopus HGF mRNA is expressed in the mesoderm region, especially in the ventral mesoderm, which for the most part gives rise to mesenchymal cells. Furthermore, HGF mRNA was expressed in response to activin A and basic FGF in blastula animal cap cells. Interestingly, a stronger activity was observed with bFGF than with activin and this finding corroborates the preferential expression of HGF mRNA in the ventral mesoderm. Based on these results, we conclude that the Xenopus homologue of HGF gene is transcribed during early embryogenesis preferentially in ventral mesodermal tissues, probably in response to the signals that induce ventral mesoderm.