Tooi O, Fujii G, Tashiro K, Shiokawa K
Laboratory of Molecular Embryology, Zoological Institute, Faculty of Science, University of Tokyo, Japan.
Biochim Biophys Acta. 1994 Sep 13;1219(1):121-8. doi: 10.1016/0167-4781(94)90254-2.
We have isolated from a Xenopus tailbud cDNA library a novel cadherin cDNA, denoted as XTCAD-1, which contained an open reading frame including the entire coding region. XTCAD-1 codes for 714 amino acids (molecular mass: 96 kDa), which include five characteristic extracellular cadherin motifs, a single putative transmembrane domain, and a cytoplasmic domain. In each domain, XTCAD-1 shared extensive homologies with other cadherins, and was related to EP-, E-, and P-cadherins more closely than to N- and M-cadherins. In adult Xenopus, XTCAD-1 mRNA was strongly expressed in intestine/stomach, kidney and skin, which are respectively derived from endoderm, mesoderm, and ectoderm. In Xenopus embryogenesis, expression of XTCAD-1 mRNA was first detected at blastula stage, and the level of the expression increased gradually during gastrula stage, reached a peak at tailbud stage and then decreased slightly at tadpole stage. These results suggest that in Xenopus laevis XTCAD-1 plays an important role in the maintenance of adult tissues that contain epithelial cells abundantly and also in morphogenesis in early embryonic development.
我们从非洲爪蟾尾芽cDNA文库中分离出一个新的钙黏蛋白cDNA，命名为XTCAD - 1，它包含一个开放阅读框，涵盖了整个编码区。XTCAD - 1编码714个氨基酸（分子量：96 kDa），其中包括五个典型的细胞外钙黏蛋白基序、一个单一的推定跨膜结构域和一个细胞质结构域。在每个结构域中，XTCAD - 1与其他钙黏蛋白具有广泛的同源性，并且与EP - 钙黏蛋白、E - 钙黏蛋白和P - 钙黏蛋白的关系比与N - 钙黏蛋白和M - 钙黏蛋白更为密切。在成年非洲爪蟾中，XTCAD - 1 mRNA在肠/胃、肾脏和皮肤中强烈表达，这些组织分别起源于内胚层、中胚层和外胚层。在非洲爪蟾胚胎发育过程中，XTCAD - 1 mRNA的表达在囊胚期首次被检测到，在原肠胚期表达水平逐渐升高，在尾芽期达到峰值，然后在蝌蚪期略有下降。这些结果表明，在非洲爪蟾中，XTCAD - 1在富含上皮细胞的成年组织的维持以及早期胚胎发育的形态发生中发挥着重要作用。
