Krammer H J, Meier-Ruge W, Sigge W, Eggers R, Kühnel W
Department of Anatomy, Medical University of Lübeck, Germany.
Eur J Pediatr Surg. 1994 Dec;4(6):358-61. doi: 10.1055/s-2008-1066134.
Neuronal intestinal dysplasia (NID) is wellknown, but its definition is a topic of debate. The histopathological diagnosis of NID is based on traditional enzyme-histochemical methods such as the acetylcholinesterase and dehydrogenase reaction on native cryosections. In this study, we have investigated the enteric nervous system in whole mount preparations of resected intestinal segments affected by NID of the plexus submucosus (type B). The plexuses of the tunica mucosa and tunica submucosa were visualized by immunohistochemical methods using a polyclonal antibody to protein gene produce 9.5 (PGP 9.5). PGP 9.5 is a novel general cytoplasmatic marker specific for the nervous system. The morphology of the plexuses is revealed in full, making possible changes easily discernible. Known pathological findings of the NID can be identified and judged more precisely with this method. Numerous enlarged nerve trunks run within the tunica submucosa and tunica mucosa. Hyperplastic ganglia with an unusually high nerve cell number in the tunica submucosa can be demonstrated as well as heterotopic nerve cells in the tunica mucosa.
神经元性肠发育异常（NID）广为人知，但其定义仍存在争议。NID的组织病理学诊断基于传统的酶组织化学方法，如对新鲜冰冻切片进行乙酰胆碱酯酶和脱氢酶反应。在本研究中，我们对受黏膜下丛（B型）NID影响的切除肠段整装标本中的肠神经系统进行了研究。使用抗蛋白基因产物9.5（PGP 9.5）的多克隆抗体，通过免疫组织化学方法观察黏膜层和黏膜下层的神经丛。PGP 9.5是一种新型的、对神经系统具有特异性的通用细胞质标记物。神经丛的形态得以完整呈现，使变化易于辨别。用这种方法可以更精确地识别和判断NID已知的病理表现。黏膜下层和黏膜层内有许多增粗的神经干。黏膜下层可见神经节增生，神经细胞数量异常增多，黏膜层也可见异位神经细胞。
