Krammer H J, Karahan S T, Sigge W, Kühnel W
Department of Anatomy, Medical University of Lübeck, Germany.
Eur J Pediatr Surg. 1994 Oct;4(5):274-8. doi: 10.1055/s-2008-1066117.
In this study we tested the immunohistochemical reactions of various markers for the enteric nervous system in whole-mount preparations of the human colon. For that purpose we used polyclonal antibodies against the neuronal markers--protein gene product 9.5 (PGP), neuron-specific enolase (NSE), neurofilament protein 200 (NFP), microtubule-associated proteins (MAPs); and the glial markers--S-100 protein and glial fibrillary acidic protein (GFAP) for the immunoperoxidase reaction. Whole-mount preparations are more suitable for histopathological evaluation and interpretation than sections, because the enteric nervous system consists of three-dimensional plexuses lying within the layers of the intestinal wall. Sections show only a part of the plexuses, neurons and glial cells. On the other hand, whole-mount preparations reveal the morphology of the plexuses as a whole. Among the neuronal and glial markers used, S-100 protein, the neurofilament protein, and the protein gene product 9.5 (PGP) produced the best results. Furthermore, this developing method provides new possibilities for the histopathological analysis of defects in the enteric nervous system, such as neuronal intestinal dysplasia (NID).
在本研究中，我们检测了人结肠整装标本中各种肠神经系统标志物的免疫组化反应。为此，我们使用了针对神经元标志物——蛋白基因产物9.5（PGP）、神经元特异性烯醇化酶（NSE）、神经丝蛋白200（NFP）、微管相关蛋白（MAPs）的多克隆抗体；以及针对神经胶质标志物——S-100蛋白和胶质纤维酸性蛋白（GFAP）的多克隆抗体，用于免疫过氧化物酶反应。整装标本比切片更适合进行组织病理学评估和解读，因为肠神经系统由位于肠壁各层内的三维神经丛组成。切片仅显示神经丛、神经元和神经胶质细胞的一部分。另一方面，整装标本能整体呈现神经丛的形态。在所使用的神经元和神经胶质标志物中，S-100蛋白、神经丝蛋白和蛋白基因产物9.5（PGP）产生了最佳结果。此外，这种改进的方法为肠神经系统缺陷（如神经元性肠发育异常，NID）的组织病理学分析提供了新的可能性。
