人胎盘生长激素重组22 kDa异构体的促生长和催乳活性。
Somatogenic and lactogenic activity of the recombinant 22 kDa isoform of human placental growth hormone.
作者信息
Igout A, Frankenne F, L'Hermite-Balériaux M, Martin A, Hennen G
机构信息
Biochimie et Laboratoire d'Endocrinologie, Université de Liège, Belgium.
出版信息
Growth Regul. 1995 Mar;5(1):60-5.
During pregnancy, the human placenta secretes a variant of pituitary growth hormone (hGH-V) differing in sequence from the pituitary growth hormone (hGH-N) by 13 amino acids substitutions. HGH-V replaces hGH-N in the maternal bloodstream during the second half of pregnancy. HGH-V is produced in two, possibly three, isoforms: a 22 kDa form, a glycosylated 25 kDa form, and a probable 26 kDa form resulting from alternative splicing of the mRNA. Here we have studied the somatogenic and lactogenic activity of recombinant 22 kDa hGH-V isoform produced in Escherichia coli and compared it with the 22 kDa form of hGH-N. The two variants exert a similar somatogenic effect on rabbit and rat cells, but differ as regards their lactogenic activity in the rat.
在怀孕期间，人胎盘会分泌一种垂体生长激素变体（hGH-V），其序列与垂体生长激素（hGH-N）有13个氨基酸的替换差异。在怀孕后半期，hGH-V会取代母体血液中的hGH-N。hGH-V以两种（可能三种）同工型产生：一种22 kDa的形式、一种糖基化的25 kDa形式，以及一种可能由mRNA可变剪接产生的26 kDa形式。在此，我们研究了在大肠杆菌中产生的重组22 kDa hGH-V同工型的促生长和催乳活性，并将其与22 kDa形式的hGH-N进行了比较。这两种变体对兔和大鼠细胞具有相似的促生长作用，但在大鼠中的催乳活性有所不同。
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