Solari A J, Dresser M E
Centro de Investigaciones en Reproduccion, Facultad de Medicina, Buenos Aires, Argentina.
Chromosome Res. 1995 Mar;3(2):87-93. doi: 10.1007/BF00710668.
The pachytene ZW pair of the chicken has been studied with a novel combination of fluorescent in situ hybridization and silver staining of the synaptonemal complexes, and with electron microscopic in situ hybridization. Probes for the EcoRI and the XhoI repeat sequences were used for light microscopy and probes for XhoI for electron microscopy. XhoI repeats are pericentromeric and correspond to 27.3% of the W axis length. EcoRI repeats form three distinct domains: domain I covers the distal 19.4% of the late-synapsing arm, and domains II and III cover respectively 21.7% and 8.5% of the W axis. The early-synapsing end containing the recombination nodule is free from any of both signals. This non-hybridizing region accounts for 25.9% of the W axis. It is suggested that this region is composed of a proximal region containing other repeat types and a terminal region which is the recombining or pseudoautosomal region. The successful combination of silver staining and fluorescent in situ hybridization will be generally useful for high-resolution localization of DNA sequences in meiotic chromosomes.
利用荧光原位杂交和联会复合体银染的新组合以及电子显微镜原位杂交技术，对鸡粗线期的ZW染色体对进行了研究。EcoRI和XhoI重复序列的探针用于光学显微镜观察，XhoI探针用于电子显微镜观察。XhoI重复序列位于着丝粒周围，占W染色体轴长的27.3%。EcoRI重复序列形成三个不同的区域：区域I覆盖后期联会臂远端的19.4%，区域II和III分别覆盖W染色体轴的21.7%和8.5%。含有重组结节的早期联会末端没有这两种信号中的任何一种。这个非杂交区域占W染色体轴的25.9%。有人认为，该区域由一个包含其他重复类型的近端区域和一个作为重组或拟常染色体区域的末端区域组成。银染和荧光原位杂交的成功结合将普遍有助于在减数分裂染色体中对DNA序列进行高分辨率定位。
