Liao Z, Allred J, Keen C L, McCune S A, Rucker R B, Medeiros D M
Department of Human Nutrition and Food Management, Ohio State University, Columbus 43201-1295, USA.
Comp Biochem Physiol B Biochem Mol Biol. 1995 May;111(1):61-7. doi: 10.1016/0305-0491(94)00226-k.
The relative amounts of cardiac proteins such as laminin, fibronectin, cytochrome c oxidase, and isomyosin types were studied by gel electrophoresis and Western blotting in control and copper-deficient Sprague-Dawley rats of both sexes fed their respective diets from weanling for 3 weeks. Isomyosin types appeared to shift from V1 to greater levels of V3 in copper deficient rats for both genders. Male copper deficient rats had increased cardiac levels of fibronectin, decreased laminin levels, cardiac hypertrophy and anemia. Both male and female rats fed copper-deficient diet had lower levels of cytochrome c oxidase (CCO) subunit IV, and low liver copper, and high heart-to-body weight ratios compared with their respective controls.
通过凝胶电泳和蛋白质免疫印迹法，研究了从断奶起分别喂食各自日粮3周的对照和缺铜的雌雄Sprague-Dawley大鼠中诸如层粘连蛋白、纤连蛋白、细胞色素c氧化酶和同工肌球蛋白类型等心脏蛋白的相对含量。缺铜大鼠无论雌雄，同工肌球蛋白类型似乎都从V1型转变为更高水平的V3型。雄性缺铜大鼠的心脏纤连蛋白水平升高、层粘连蛋白水平降低、心脏肥大且贫血。与各自的对照组相比，喂食缺铜日粮的雌雄大鼠细胞色素c氧化酶（CCO）亚基IV水平较低、肝脏铜含量低且心脏与体重比值高。
