Jalili T, Medeiros D M, Wildman R E
Department of Human Nutrition and Food Management, Ohio State University, Columbus 43210, USA.
J Nutr. 1996 Apr;126(4):807-16. doi: 10.1093/jn/126.4.807.
The objective of this study was to determine if a high fat diet having a 2:1 saturated-polyunsaturated fatty acid ratio exacerbates signs of copper deficiency. Male weanling Long-Evans rats were randomly placed into one of the following treatment groups: adequate copper low fat or deficient copper high fat. The levels of fat used were 31 or 12% of daily energy, and copper concentrations were 94.5 micromol/kg and <15.8 micromol/kg in the copper-adequate and copper-deficient diets, respectively. Cardiac hypertrophy as well as lower liver copper levels and superoxide dismutase activity were observed in both groups of copper-deficient rats. Irrespective of copper level, consumption of the high fat diet resulted in the thickening of the interventricular septum and left ventricular free wall. Electrocardiograms revealed that the copper-deficient high fat diet led to a significantly smaller QT interval compared with all other groups. Significantly greater S-wave voltage due to copper deficiency was observed. Significantly lower heart cytochrome c oxidase (CCO) activity was found in the copper-deficient groups with the copper deficient high fat group showing the lowest activity. Western blots of the cardiac non-myofibrillar fraction demonstrated lower amounts of CCO nuclear encoded peptides in the copper-deficient groups, with the least amount seen in the copper-deficient high fat treatment. These data suggest that a high level of dietary fat exacerbates some of the signs of copper deficiency.
本研究的目的是确定饱和脂肪酸与多不饱和脂肪酸比例为2:1的高脂饮食是否会加剧铜缺乏的症状。雄性断奶Long-Evans大鼠被随机分为以下治疗组之一：充足铜低脂组或缺乏铜高脂组。所用脂肪水平分别为每日能量的31%或12%，铜充足组和铜缺乏组饮食中的铜浓度分别为94.5微摩尔/千克和<15.8微摩尔/千克。两组铜缺乏大鼠均观察到心脏肥大以及肝脏铜水平降低和超氧化物歧化酶活性降低。无论铜水平如何，高脂饮食的摄入都会导致室间隔和左心室游离壁增厚。心电图显示，与所有其他组相比，铜缺乏高脂饮食导致QT间期明显缩短。观察到由于铜缺乏导致S波电压明显升高。在铜缺乏组中发现心脏细胞色素c氧化酶（CCO）活性明显降低，其中铜缺乏高脂组活性最低。心脏非肌原纤维部分的蛋白质免疫印迹显示，铜缺乏组中CCO核编码肽的含量较低，铜缺乏高脂治疗组中含量最少。这些数据表明，高水平的膳食脂肪会加剧铜缺乏的一些症状。
