结直肠癌中鸟氨酸脱羧酶的免疫组织化学染色及活性

Immunohistochemical staining and activity of ornithine decarboxylase in colorectal cancer.

作者信息

Oya K, Yamane T, Inagake M, Kitao Y, Okuyama A, Takahashi T, Irie S, Nakayama K, Wadama K

机构信息

First Department of Surgery, Kyoto Prefectural University of Medicine, Japan.

出版信息

Cancer Lett. 1995 May 4;91(1):101-6. doi: 10.1016/0304-3835(94)03726-y.

DOI:10.1016/0304-3835(94)03726-y

PMID:7750083

Abstract

Using a new anti-human ornithine decarboxylase (anti-hODC) monoclonal antibody, the relationship between the immunoreactivity of ODC and its activity was analyzed in 21 human colorectal cancer tissues, 42 adjacent non-tumorous mucosa specimens, and 10 normal rectal mucosa samples from frozen sections and paraffin-embedded samples. A statistical significant correlation was found between the antibody reaction and the enzymic activity (P < 0.01). The immunohistochemical staining for ODC provides a new and simplified procedure for studying the activity of ODC as compared to previous methods using radioisotopes. It offers the advantages of retrospectively determining the amount of ODC in samples previously embedded in paraffin.

摘要

使用一种新的抗人鸟氨酸脱羧酶（抗hODC）单克隆抗体，对21例人大肠癌组织、42例相邻非肿瘤黏膜标本以及10例来自冰冻切片和石蜡包埋样本的正常直肠黏膜样本中ODC的免疫反应性与其活性之间的关系进行了分析。发现抗体反应与酶活性之间存在统计学显著相关性（P<0.01）。与以往使用放射性同位素的方法相比，ODC的免疫组织化学染色为研究ODC的活性提供了一种新的简化程序。它具有可追溯性地测定先前石蜡包埋样本中ODC含量的优点。