Pankow W, Neumann K, Rüschoff J, von Wichert P
Department of Internal Medicine, University Hospital, Philipps University, Marburg, Germany.
Cancer Detect Prev. 1995;19(3):268-73.
To investigate the influence of cigarette smoking on alveolar macrophages (AM) we compared morphologic and immunocytochemic characteristics of bronchoalveolar lavage cells collected from 10 smokers and 8 nonsmokers. AM were studied using single cell fluorescence photometry. AM of smokers were larger in size (p < 0.001), showed stronger autofluorescence (p < 0.001), and expressed fewer HLA-DR antigens (p < 0.001) compared with AM of nonsmokers. AM of smokers were more heterogeneous in respect to cell size, autofluorescence, and HLA-DR antigen expression. HLA-DR antigen density on AM of smokers was independent of cell size and autofluorescence. This suggests that tobacco smoke reduces HLA-DR antigen expression in AM of different maturational states and that this defective expression of HLA class II antigens is not dependent on the amount of phagocytosed autofluorescent tobacco particles.
为研究吸烟对肺泡巨噬细胞（AM）的影响，我们比较了从10名吸烟者和8名不吸烟者收集的支气管肺泡灌洗细胞的形态学和免疫细胞化学特征。使用单细胞荧光光度法研究AM。与不吸烟者的AM相比，吸烟者的AM体积更大（p < 0.001），自发荧光更强（p < 0.001），且表达的HLA - DR抗原更少（p < 0.001）。吸烟者的AM在细胞大小、自发荧光和HLA - DR抗原表达方面更具异质性。吸烟者AM上的HLA - DR抗原密度与细胞大小和自发荧光无关。这表明烟草烟雾会降低不同成熟状态的AM中HLA - DR抗原的表达，且这种HLA II类抗原的缺陷表达不依赖于吞噬的自发荧光烟草颗粒的数量。
