Rasschaert J, Reusens B, Dahri S, Sener A, Remacle C, Hoet J J, Malaisse W J
Laboratory of Experimental Medicine, Erasmus Medical School, Brussels Free University, Belgium.
Endocrinology. 1995 Jun;136(6):2631-4. doi: 10.1210/endo.136.6.7750486.
In rats that received a low protein isocaloric diet (protein content of the diet: 8 instead of 20%) during fetal life and thereafter up to the time of sacrifice at 12-13 weeks of age, a low plasma insulin concentration, a decreased insulin content of isolated pancreatic islets, and an impaired secretory response of the islets to either D-glucose or the association of L-leucine and L-glutamine coincided, in islet homogenates, with a low activity of the mitochondrial glycerophosphate dehydrogenase and an abnormally high ratio between glutamate-alanine and glutamate-aspartate transaminase activities. Opposite enzymatic changes were found in liver extracts of the same rats. No obvious change in these hormonal, secretory, and enzymatic variables were observed when the period of protein deficiency was restricted to fetal life. These findings support the view that, in protein malnutrition, an impaired activity of pancreatic B-cell mitochondrial glycerophosphate dehydrogenase contributes, possibly in association with other enzymatic anomalies, to the perturbation of islet function.
在胎儿期接受低蛋白等热量饮食（饮食中的蛋白质含量：8%而非20%），并在12 - 13周龄处死前一直维持该饮食的大鼠中，血浆胰岛素浓度低、分离的胰岛中胰岛素含量降低、胰岛对D - 葡萄糖或L - 亮氨酸与L - 谷氨酰胺组合的分泌反应受损，在胰岛匀浆中，同时存在线粒体甘油磷酸脱氢酶活性低以及谷氨酸 - 丙氨酸转氨酶与谷氨酸 - 天冬氨酸转氨酶活性之间的异常高比值。在相同大鼠的肝脏提取物中发现了相反的酶变化。当蛋白质缺乏期仅限于胎儿期时，未观察到这些激素、分泌和酶变量有明显变化。这些发现支持这样一种观点，即在蛋白质营养不良中，胰腺β细胞线粒体甘油磷酸脱氢酶活性受损，可能与其他酶异常相关，导致胰岛功能紊乱。
