Henderson I, Yu D, Turnbull P C
Centre for Applied Microbiology, Salisbury, Wiltshire, UK.
FEMS Microbiol Lett. 1995 May 1;128(2):113-8. doi: 10.1111/j.1574-6968.1995.tb07509.x.
Sequences based on the conserved 20 bp inverted repeat of IS231 variants were used as polymerase chain reaction-based fingerprinting primers of the member species of the Bacillus cereus group (B. anthracis, B. cereus, B. thuringiensis and B. mycoides), because of their close association with transposons, principally Tn4430 in B. thuringiensis. Fingerprints of B. anthracis were simple, and specifically allowed its identification and sub-differentiation from other members of the group. Fingerprints for B. cereus were strain-specific; those for B. thuringensis gave a 1650 bp product, characteristic of IS231 variants A-F. The same reaction conditions gave one or two bands for both B. anthracis and B. cereus that differed by restriction endonuclease mapping from the B. thuringiensis PCR product and established IS231 restriction maps; this does not preclude some kind of relationship between these products and IS231.
基于IS231变体保守的20 bp反向重复序列的片段被用作蜡样芽孢杆菌群（炭疽芽孢杆菌、蜡样芽孢杆菌、苏云金芽孢杆菌和蕈状芽孢杆菌）成员物种基于聚合酶链反应的指纹图谱引物，因为它们与转座子密切相关，主要是苏云金芽孢杆菌中的Tn4430。炭疽芽孢杆菌的指纹图谱简单，可特异性地对其进行鉴定并与该菌群的其他成员区分开来。蜡样芽孢杆菌的指纹图谱具有菌株特异性；苏云金芽孢杆菌的指纹图谱产生一个1650 bp的产物，这是IS231变体A - F的特征。相同的反应条件下，炭疽芽孢杆菌和蜡样芽孢杆菌均产生一或两条条带，通过限制性内切酶图谱分析，这些条带与苏云金芽孢杆菌PCR产物不同，并建立了IS231限制性图谱；这并不排除这些产物与IS231之间存在某种关系。
