Daffonchio D, Borin S, Frova G, Gallo R, Mori E, Fani R, Sorlini C
Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Università degli Studi di Milano, 20133 Milan, Italy.
Appl Environ Microbiol. 1999 Mar;65(3):1298-303. doi: 10.1128/AEM.65.3.1298-1303.1999.
Aiming to develop a DNA marker specific for Bacillus anthracis and able to discriminate this species from Bacillus cereus, Bacillus thuringiensis, and Bacillus mycoides, we applied the randomly amplified polymorphic DNA (RAPD) fingerprinting technique to a collection of 101 strains of the genus Bacillus, including 61 strains of the B. cereus group. An 838-bp RAPD marker (SG-850) specific for B. cereus, B. thuringiensis, B. anthracis, and B. mycoides was identified. This fragment included a putative (366-nucleotide) open reading frame highly homologous to the ypuA gene of Bacillus subtilis. The restriction analysis of the SG-850 fragment with AluI distinguished B. anthracis from the other species of the B. cereus group.
为了开发一种针对炭疽芽孢杆菌的DNA标记，并能够将该物种与蜡样芽孢杆菌、苏云金芽孢杆菌和蕈状芽孢杆菌区分开来，我们将随机扩增多态性DNA（RAPD）指纹技术应用于101株芽孢杆菌属菌株，其中包括61株蜡样芽孢杆菌组菌株。鉴定出了一种针对蜡样芽孢杆菌、苏云金芽孢杆菌、炭疽芽孢杆菌和蕈状芽孢杆菌的838 bp RAPD标记（SG-850）。该片段包含一个与枯草芽孢杆菌ypuA基因高度同源的推定（366个核苷酸）开放阅读框。用AluI对SG-850片段进行限制性分析，可将炭疽芽孢杆菌与蜡样芽孢杆菌组的其他物种区分开来。
