一种对蜡样芽孢杆菌群特异的随机扩增多态性DNA标记物可用于炭疽芽孢杆菌的诊断。
A randomly amplified polymorphic DNA marker specific for the Bacillus cereus group is diagnostic for Bacillus anthracis.
作者信息
Daffonchio D, Borin S, Frova G, Gallo R, Mori E, Fani R, Sorlini C
机构信息
Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Università degli Studi di Milano, 20133 Milan, Italy.
出版信息
Appl Environ Microbiol. 1999 Mar;65(3):1298-303. doi: 10.1128/AEM.65.3.1298-1303.1999.
Abstract
Aiming to develop a DNA marker specific for Bacillus anthracis and able to discriminate this species from Bacillus cereus, Bacillus thuringiensis, and Bacillus mycoides, we applied the randomly amplified polymorphic DNA (RAPD) fingerprinting technique to a collection of 101 strains of the genus Bacillus, including 61 strains of the B. cereus group. An 838-bp RAPD marker (SG-850) specific for B. cereus, B. thuringiensis, B. anthracis, and B. mycoides was identified. This fragment included a putative (366-nucleotide) open reading frame highly homologous to the ypuA gene of Bacillus subtilis. The restriction analysis of the SG-850 fragment with AluI distinguished B. anthracis from the other species of the B. cereus group.
摘要
为了开发一种针对炭疽芽孢杆菌的DNA标记，并能够将该物种与蜡样芽孢杆菌、苏云金芽孢杆菌和蕈状芽孢杆菌区分开来，我们将随机扩增多态性DNA（RAPD）指纹技术应用于101株芽孢杆菌属菌株，其中包括61株蜡样芽孢杆菌组菌株。鉴定出了一种针对蜡样芽孢杆菌、苏云金芽孢杆菌、炭疽芽孢杆菌和蕈状芽孢杆菌的838 bp RAPD标记（SG-850）。该片段包含一个与枯草芽孢杆菌ypuA基因高度同源的推定（366个核苷酸）开放阅读框。用AluI对SG-850片段进行限制性分析，可将炭疽芽孢杆菌与蜡样芽孢杆菌组的其他物种区分开来。
相似文献
1
A randomly amplified polymorphic DNA marker specific for the Bacillus cereus group is diagnostic for Bacillus anthracis.一种对蜡样芽孢杆菌群特异的随机扩增多态性DNA标记物可用于炭疽芽孢杆菌的诊断。
Appl Environ Microbiol. 1999 Mar;65(3):1298-303. doi: 10.1128/AEM.65.3.1298-1303.1999.
2
Generation of a specific marker to discriminate Gacillus anthracis from other bacteria of the Bacillus cereus group.生成一种用于区分炭疽芽孢杆菌与蜡样芽孢杆菌群其他细菌的特异性标志物。
J Microbiol Biotechnol. 2007 May;17(5):806-11.
3
Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates.炭疽芽孢杆菌、蜡样芽孢杆菌和苏云金芽孢杆菌分离株的荧光扩增片段长度多态性分析
Appl Environ Microbiol. 2004 Feb;70(2):1068-80. doi: 10.1128/AEM.70.2.1068-1080.2004.
4
Glycosyltransferase: a specific marker for the discrimination of Bacillus anthracis from the Bacillus cereus group.糖基转移酶：用于区分炭疽芽孢杆菌与蜡样芽孢杆菌群的特异性标志物。
J Med Microbiol. 2008 Mar;57(Pt 3):279-286. doi: 10.1099/jmm.0.47642-0.
5
Identification of a Bacillus anthracis specific indel in the yeaC gene and development of a rapid pyrosequencing assay for distinguishing B. anthracis from the B. cereus group.鉴定炭疽芽孢杆菌 yeaC 基因中的插入缺失并开发一种快速焦磷酸测序检测方法，用于区分炭疽芽孢杆菌和芽孢杆菌属。
J Microbiol Methods. 2011 Dec;87(3):278-85. doi: 10.1016/j.mimet.2011.08.015. Epub 2011 Sep 2.
6
Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis.区分炭疽芽孢杆菌与蜡样芽孢杆菌和苏云金芽孢杆菌的基因组差异。
Appl Environ Microbiol. 2003 May;69(5):2755-64. doi: 10.1128/AEM.69.5.2755-2764.2003.
7
Differentiation of Bacillus anthracis, B. cereus, and B. thuringiensis by using pulsed-field gel electrophoresis.运用脉冲场凝胶电泳技术对炭疽芽孢杆菌、蜡样芽孢杆菌和苏云金芽孢杆菌进行鉴别。
Appl Environ Microbiol. 2007 May;73(10):3446-9. doi: 10.1128/AEM.02478-06. Epub 2007 Mar 30.
8
Identification of strain specific markers in Bacillus anthracis by random amplification of polymorphic DNA.通过多态性DNA的随机扩增鉴定炭疽芽孢杆菌中的菌株特异性标记。
FEMS Microbiol Lett. 2005 Mar 1;244(1):199-205. doi: 10.1016/j.femsle.2005.01.039.
9
Discrimination of the Bacillus cereus group members by pattern analysis of random amplified polymorphic DNA-PCR.通过随机扩增多态性DNA-PCR的图谱分析鉴别蜡样芽孢杆菌群成员
Biocontrol Sci. 2012 Jun;17(2):83-6. doi: 10.4265/bio.17.83.
10
Identification of Bacillus anthracis by multiprobe microarray hybridization.通过多探针微阵列杂交鉴定炭疽芽孢杆菌。
Diagn Microbiol Infect Dis. 2004 Jul;49(3):163-71. doi: 10.1016/j.diagmicrobio.2004.03.015.
引用本文的文献
1
Rapid Identification of Bacillus anthracis and On-Site Using Novel Single-Nucleotide Polymorphisms.利用新型单核苷酸多态性快速鉴定炭疽芽孢杆菌和现场检测
Microbiol Spectr. 2022 Jun 29;10(3):e0228521. doi: 10.1128/spectrum.02285-21. Epub 2022 May 16.
2
Detection and Identification of : From Conventional to Molecular Microbiology Methods.检测与鉴定：从传统微生物学方法到分子微生物学方法
Microorganisms. 2020 Jan 16;8(1):125. doi: 10.3390/microorganisms8010125.
3
Identification of Bacillus species: Implication on the quality of probiotic formulations.芽孢杆菌属的鉴定：对益生菌制剂质量的影响。
PLoS One. 2019 May 20;14(5):e0217021. doi: 10.1371/journal.pone.0217021. eCollection 2019.
4
Requirements for the Development of Bacillus Anthracis Spore Reference Materials Used to Test Detection Systems.用于检测系统测试的炭疽芽孢杆菌孢子参考物质的研制要求。
J Res Natl Inst Stand Technol. 2006 Jun 1;111(3):205-17. doi: 10.6028/jres.111.017. Print 2006 May-Jun.
5
In silico and in vitro evaluation of PCR-based assays for the detection of Bacillus anthracis chromosomal signature sequences.基于 PCR 的炭疽杆菌染色体特征序列检测分析方法的计算机模拟和体外评估。
Virulence. 2013 Nov 15;4(8):671-85. doi: 10.4161/viru.26288. Epub 2013 Sep 9.
6
Bacillus "next generation" diagnostics: moving from detection toward subtyping and risk-related strain profiling.芽孢杆菌“下一代”诊断：从检测转向亚型和与风险相关的菌株分析。
Front Microbiol. 2013 Feb 22;4:32. doi: 10.3389/fmicb.2013.00032. eCollection 2013.
7
Molecular identification of hemolymph-associated symbiotic bacteria in red imported fire ant larvae.红火蚁幼虫血淋巴相关共生细菌的分子鉴定
Curr Microbiol. 2008 Dec;57(6):575-9. doi: 10.1007/s00284-008-9245-2. Epub 2008 Sep 16.
8
Diversity of Bacillus anthracis strains in Georgia and of vaccine strains from the former Soviet Union.格鲁吉亚炭疽芽孢杆菌菌株及前苏联疫苗菌株的多样性
Appl Environ Microbiol. 2006 Aug;72(8):5631-6. doi: 10.1128/AEM.00440-06.
9
Characterization of Bacillus anthracis-like bacteria isolated from wild great apes from Cote d'Ivoire and Cameroon.从科特迪瓦和喀麦隆野生大猩猩中分离出的炭疽芽孢杆菌样细菌的特性研究。
J Bacteriol. 2006 Aug;188(15):5333-44. doi: 10.1128/JB.00303-06.
10
Some Bacillus thuringiensis strains share rpoB nucleotide polymorphisms also present in Bacillus anthracis.一些苏云金芽孢杆菌菌株具有与炭疽芽孢杆菌相同的rpoB核苷酸多态性。
J Clin Microbiol. 2006 Apr;44(4):1606-7. doi: 10.1128/JCM.44.4.1606-1607.2006.
本文引用的文献
1
A bacterial toxin paralysing silkworm larvae.一种可使蚕幼虫麻痹的细菌毒素。
Nature. 1954 Mar 20;173(4403):545-6. doi: 10.1038/173545a0.
2
Molecular characterization of Bacillus strains involved in outbreaks of anthrax in France in 1997.1997年法国炭疽疫情中涉及的芽孢杆菌菌株的分子特征分析
J Clin Microbiol. 1998 Nov;36(11):3412-4. doi: 10.1128/JCM.36.11.3412-3414.1998.
3
16S-23S rRNA internal transcribed spacers as molecular markers for the species of the 16S rRNA group I of the genus Bacillus.16S-23S rRNA内部转录间隔区作为芽孢杆菌属16S rRNA第一组物种的分子标记。
FEMS Microbiol Lett. 1998 Jun 15;163(2):229-36. doi: 10.1111/j.1574-6968.1998.tb13050.x.
4
PCR fingerprinting of whole genomes: the spacers between the 16S and 23S rRNA genes and of intergenic tRNA gene regions reveal a different intraspecific genomic variability of Bacillus cereus and Bacillus licheniformis [corrected].全基因组的PCR指纹图谱：16S和23S rRNA基因之间的间隔区以及基因间tRNA基因区域揭示了蜡样芽孢杆菌和地衣芽孢杆菌不同的种内基因组变异性[已修正] 。
Int J Syst Bacteriol. 1998 Jan;48 Pt 1:107-16. doi: 10.1099/00207713-48-1-107.
5
Single strand conformation polymorphism analysis of PCR-tDNA fingerprinting to address the identification of Bacillus species.采用PCR - tDNA指纹图谱的单链构象多态性分析来鉴定芽孢杆菌属菌种。
FEMS Microbiol Lett. 1997 Dec 1;157(1):87-93. doi: 10.1111/j.1574-6968.1997.tb12757.x.
6
Characterization of the variable-number tandem repeats in vrrA from different Bacillus anthracis isolates.不同炭疽芽孢杆菌分离株中vrrA基因可变数目串联重复序列的特征分析
Appl Environ Microbiol. 1997 Apr;63(4):1400-5. doi: 10.1128/aem.63.4.1400-1405.1997.
7
Use of an arbitrarily primed PCR product in the development of a Campylobacter jejuni-specific PCR.在空肠弯曲菌特异性聚合酶链反应（PCR）的开发中使用任意引物PCR产物。
Appl Environ Microbiol. 1997 Mar;63(3):1019-23. doi: 10.1128/aem.63.3.1019-1023.1997.
8
Identification and characterization of Bacillus anthracis by multiplex PCR analysis of sequences on plasmids pXO1 and pXO2 and chromosomal DNA.通过对质粒pXO1和pXO2以及染色体DNA上的序列进行多重PCR分析来鉴定和表征炭疽芽孢杆菌。
FEMS Microbiol Lett. 1996 Nov 15;145(1):9-16. doi: 10.1111/j.1574-6968.1996.tb08548.x.
9
Isolation of a specific chromosomic DNA sequence of Bacillus anthracis and its possible use in diagnosis.炭疽芽孢杆菌特定染色体DNA序列的分离及其在诊断中的可能应用。
FEMS Immunol Med Microbiol. 1996 Oct;15(4):223-31. doi: 10.1111/j.1574-695X.1996.tb00088.x.
10
The use of RAPD for generating specific DNA probes for microorganisms.随机扩增多态性DNA技术用于生成微生物特异性DNA探针。
Methods Mol Biol. 1996;50:155-75. doi: 10.1385/0-89603-323-6:155.
Zotero 插件