Williams A O, Ward J M, Li J F, Jackson M A, Flanders K C
Laboratory of Experimental Pathology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Hum Pathol. 1995 May;26(5):469-73. doi: 10.1016/0046-8177(95)90241-4.
Immunohistochemical localization of transforming growth factor beta 1 (TGF-beta 1) was studied in Kaposi's sarcoma (KS) tissues obtained from autopsy and biopsy materials of patients with and without acquired immunodeficiency syndrome (AIDS) or human immunodeficiency virus (HIV) infection. There was no difference in the localization and distribution of TGF-beta 1 in KS tissues regardless of the HIV-1 status of the patients. Rabbit polyclonal antibodies to synthetic peptides, corresponding to the first 30 amino acids of mature TGF-beta 1, anti-LC(1-30), and anti-CC(1-30), were used for localization of intracellular and extracellular TGF-beta 1. An antibody to a peptide corresponding to amino acids 266 to 278 of the TGF-beta 1 precursor sequence anti-Pre(266 to 278) was used to detect the TGF-beta 1 precursor and the latency-associated peptide. Intracellular mature TGF-beta 1 was demonstrated in mononuclear cells, presumably macrophages, within KS tumors but not in spindle-shaped KS cells. Extracellular mature TGF-beta 1 was localized in the basement membranes of blood vessels and fibrous capsules of KS tumors. Intracellular reactivity to anti-Pre was localized in vascular smooth muscle cells and pericytes within the tumor, in variable proportions of spindle-shaped KS cells, and also in macrophage-like cells. These cells appear to be the production sites of TGF-beta 1, which may exert paracrine as well as autocrine proliferative effects.
在从患有和未患获得性免疫缺陷综合征（AIDS）或人类免疫缺陷病毒（HIV）感染的患者尸检和活检材料中获取的卡波西肉瘤（KS）组织中，研究了转化生长因子β1（TGF-β1）的免疫组织化学定位。无论患者的HIV-1状态如何，TGF-β1在KS组织中的定位和分布均无差异。针对与成熟TGF-β1的前30个氨基酸相对应的合成肽的兔多克隆抗体，即抗-LC(1-30)和抗-CC(1-30)，用于细胞内和细胞外TGF-β1的定位。针对与TGF-β1前体序列的第266至278个氨基酸相对应的肽的抗体抗-Pre(266至278)，用于检测TGF-β1前体和潜伏期相关肽。在KS肿瘤内的单核细胞（可能是巨噬细胞）中证实了细胞内成熟TGF-β1，但在梭形KS细胞中未证实。细胞外成熟TGF-β1定位于KS肿瘤血管的基底膜和纤维囊。抗-Pre的细胞内反应性定位于肿瘤内的血管平滑肌细胞和周细胞、不同比例的梭形KS细胞以及巨噬细胞样细胞中。这些细胞似乎是TGF-β1的产生部位，TGF-β1可能发挥旁分泌以及自分泌增殖作用。
