Roth W K
Chemotherapeutisches Forschungsinstitut Georg-Speyer-Haus, Frankfurt/Main, Germany.
Res Virol. 1993 Jan-Feb;144(1):105-9. doi: 10.1016/s0923-2516(06)80019-1.
Conditioned media from cell cultures derived from AIDS-associated Kaposi's sarcoma (KS) promote the growth of KS cells, fibroblasts and endothelial cells. Application of KS cells to the chicken chorion allantoic membrane induces neo-angiogenesis similar to that caused by fibroblast growth factors (FGF). Testing for known factors of the FGF family by Northern blotting does not reveal any difference from control fibroblasts negative for such an activity in their supernatants. This indicates secretion of an unknown FGF-like factor by KS cells possessing autocrine (KS cells) and paracrine (fibroblasts, endothelial cells) activity. It was reported that transforming growth factor-beta (TGF beta) is secreted by HIV-infected lymphocytes in AIDS patients. By testing this growth factor on KS cell cultures, growth-promoting activity was observed 5 to 20 times greater than that of the control value without TGF beta.
来自艾滋病相关卡波西肉瘤（KS）细胞培养物的条件培养基可促进KS细胞、成纤维细胞和内皮细胞的生长。将KS细胞应用于鸡绒毛尿囊膜可诱导新血管生成，类似于成纤维细胞生长因子（FGF）所引起的情况。通过Northern印迹法检测FGF家族的已知因子，未发现其与上清液中对此活性呈阴性的对照成纤维细胞有任何差异。这表明具有自分泌（KS细胞）和旁分泌（成纤维细胞、内皮细胞）活性的KS细胞分泌了一种未知的FGF样因子。据报道，艾滋病患者中受HIV感染的淋巴细胞会分泌转化生长因子-β（TGF-β）。通过在KS细胞培养物上测试这种生长因子，观察到其促生长活性比无TGF-β时的对照值高5至20倍。
