Xu D, Liew F Y
Department of Immunology, University of Glasgow, UK.
Immunology. 1995 Feb;84(2):173-6.
cDNA encoding the highly conserved major surface glycoprotein, gp63, of Leishmania major was cloned, together with a signal sequence, into an eukaryotic expression vector, pCDNAI, which carries the human cytomegalovirus (CMV) promoter. This construct, pCMV/glycoprotein 63 (gp63), when injected into the skeletal muscle of BALB/c mice expressed sustained levels of gp63 in the muscle tissue for at least 40 days. Spleen and lymph node cells from the immunized mice produced significant amounts of interleukin-2 (IL-2) and interferon-gamma (IFN-gamma) but no detectable IL-4 when cultured with L. major antigens in vitro. The immunized mice also developed significant resistance against L. major infection compared to control mice injected with the empty plasmid. These results suggest that nucleic acid vaccine is effective against parasite infections.
编码利什曼原虫主要表面糖蛋白gp63的cDNA，连同信号序列一起被克隆到一个携带人巨细胞病毒（CMV）启动子的真核表达载体pCDNAI中。该构建体pCMV/糖蛋白63（gp63）注射到BALB/c小鼠的骨骼肌中后，在肌肉组织中持续表达gp63至少40天。免疫小鼠的脾细胞和淋巴结细胞在体外与利什曼原虫主要抗原一起培养时，产生了大量的白细胞介素-2（IL-2）和干扰素-γ（IFN-γ），但未检测到IL-4。与注射空质粒的对照小鼠相比，免疫小鼠对利什曼原虫主要感染也产生了显著的抵抗力。这些结果表明核酸疫苗对寄生虫感染有效。
