Weiss C, Kinscherf R, Roth S, Friedmann B, Fischbach T, Reus J, Dröge W, Bärtsch P
Medical Clinic and Policlinic, Department of Sports and Performance Medicine, Heidelberg, Germany.
Int J Sports Med. 1995 Feb;16(2):117-21. doi: 10.1055/s-2007-972976.
In 13 middle-aged, moderately trained men (40-60 yr) we investigated the influence of anaerobic training on immunological parameters measured at rest. The 4 week anaerobic training program (two 30-min sessions weight lifting and one interval training per week; lactate levels 4-6 mM and 8-10 mM, respectively), caused a significant increase of the mean arm muscle force by 7% (handgrip test, p < 0.05). Evaluation of lymphocyte subsets was performed by means of three-colour immunofluorescence analysis (FACS). After 4 weeks of training we found a significant reduction of the CD4+ T-cell counts by 15% (p < 0.05) paralleled by a fall of naive cells (CD3+/CD4+/CD45RA+) by 16%, which, however, was statistically not significant. While percentages of CD3+ lymphocytes decreased significantly by 6% (p < 0.001), absolute numbers of CD3+ T-lymphocytes were not detectably affected and also the relative ratio of CD8+ T-cell subsets, i.e. the ratio of suppressor vs cytotoxic T-cells (CD3+/CD8+/CD11b+, CD3+/CD8+/CD11b- respectively) remained unchanged. Likewise the serum concentrations of the soluble CD8 and CD4 antigen (sCD8/sCD4) as determined by sandwich enzyme immunoassays were found to be unaffected. We conclude that 40-60 years old healthy human subjects performing anaerobic training experience on average a significant decrease of circulating CD4+ T-lymphocytes, while other parameters including the activation parameters sCD8 and sCD4 remained unchanged.
在13名中年、适度训练的男性（40 - 60岁）中，我们研究了无氧训练对静息时所测免疫参数的影响。为期4周的无氧训练计划（每周两次30分钟的举重训练和一次间歇训练；乳酸水平分别为4 - 6 mM和8 - 10 mM）使平均手臂肌肉力量显著增加了7%（握力测试，p < 0.05）。通过三色免疫荧光分析（FACS）对淋巴细胞亚群进行评估。训练4周后，我们发现CD4 + T细胞计数显著减少了15%（p < 0.05），同时初始细胞（CD3 + /CD4 + /CD45RA +）减少了16%，不过这在统计学上并不显著。虽然CD3 +淋巴细胞百分比显著下降了6%（p < 0.001），但CD3 + T淋巴细胞的绝对数量未受明显影响，而且CD8 + T细胞亚群的相对比例，即抑制性T细胞与细胞毒性T细胞的比例（分别为CD3 + /CD8 + /CD11b +、CD3 + /CD8 + /CD11b -）保持不变。同样，通过夹心酶免疫测定法测定的可溶性CD8和CD4抗原的血清浓度也未受影响。我们得出结论，进行无氧训练的40 - 60岁健康人类受试者平均循环CD4 + T淋巴细胞显著减少，而包括激活参数sCD8和sCD4在内的其他参数保持不变。
