Kajiura J S, MacDougall J D, Ernst P B, Younglai E V
Department of Kinesiology, McMaster University, Hamilton, Ontario, Canada.
Med Sci Sports Exerc. 1995 Aug;27(8):1111-7.
We examined the acute and chronic effects of changes in training volume and intensity on the blood lymphocyte percentages and immunoglobulin levels in runners. Twelve runners participated in four 10-d phases of low volume/low intensity (LV/LI), high volume/low intensity (HV/LI), or high volume/high intensity (HV/HI) running. Subjects were assigned to one of two different training group orders: 1) LV/LI, HV/LI, LV/LI, HV/HI; or 2) LV/LI, HV/HI, LV/LI, HV/LI. Venous blood was drawn at rest on days 1, 4, and 7; and 5 min post-exercise on days 1 and 7 of each 10-d phase. Lymphocyte subsets were determined by flow cytometry for CD3+, CD4+, CD8+, and HLA-DR+. IgG, and IgM levels were obtained by ELISA analysis. Immunoglobulin, CD8+ and HLA-DR+ levels, and pre-exercise plasma cortisol concentrations were not significantly affected by alterations in volume or intensity. A transient decrease (P < 0.05) was observed in CD4+ and the CD4/CD8 ratio 5 min post-exercise during the HV/LI and HV/HI phases. Results indicate that the exercise-induced lymphocyte subset reduction is transient and suggest that it is more dependent upon training intensity than volume, and the training order of exposure to the high-intensity stimulus may determine the magnitude of subsequent responses.
我们研究了训练量和强度的变化对跑步者血液淋巴细胞百分比和免疫球蛋白水平的急性和慢性影响。12名跑步者参加了四个为期10天的低量/低强度（LV/LI）、高量/低强度（HV/LI）或高量/高强度（HV/HI）跑步阶段。受试者被分配到两种不同的训练组顺序之一：1）LV/LI、HV/LI、LV/LI、HV/HI；或2）LV/LI、HV/HI、LV/LI、HV/LI。在每个10天阶段的第1、4和7天休息时以及第1和7天运动后5分钟采集静脉血。通过流式细胞术测定CD3 +、CD4 +、CD8 +和HLA-DR +的淋巴细胞亚群。通过ELISA分析获得IgG和IgM水平。免疫球蛋白、CD8 +和HLA-DR +水平以及运动前血浆皮质醇浓度不受量或强度变化的显著影响。在HV/LI和HV/HI阶段运动后5分钟观察到CD4 +和CD4/CD8比值短暂下降（P < 0.05）。结果表明，运动引起的淋巴细胞亚群减少是短暂的，表明它更多地取决于训练强度而非训练量，并且暴露于高强度刺激的训练顺序可能决定随后反应的幅度。
