在大肠杆菌中表达的猪肺炎支原体重组46千道尔顿表面抗原（P46）可用于通过酶联免疫吸附测定法对猪支原体肺炎进行早期特异性诊断。
Recombinant 46-kilodalton surface antigen (P46) of Mycoplasma hyopneumoniae expressed in Escherichia coli can be used for early specific diagnosis of mycoplasmal pneumonia of swine by enzyme-linked immunosorbent assay.
作者信息
Futo S, Seto Y, Okada M, Sato S, Suzuki T, Kawai K, Imada Y, Mori Y
机构信息
United Graduate School of Agricultural Sciences, Gifu University, Japan.
出版信息
J Clin Microbiol. 1995 Mar;33(3):680-3. doi: 10.1128/jcm.33.3.680-683.1995.
Abstract
The 46-kDa surface antigen (P46) is the early and species-specific immunogenic protein of Mycoplasma hyopneumoniae. Three TGA codons encoding tryptophan in the P46 gene were replaced with TGG by an in vitro mutagenesis technique. The mutated P46 gene was expressed in Escherichia coli by using the chelating peptide tag system. The purified recombinant P46 was successfully used in an enzyme-linked immunosorbent assay for detection of antibodies against M. hyopneumoniae in swine serum. It did not cross-react with sera from swine infected with Mycoplasma flocculate, Mycoplasma hyorhinis, or Mycoplasma hyosynoviae. With this method, mycoplasmal pneumonia of swine was detectable within 2 weeks after infection.
摘要
46 kDa表面抗原（P46）是猪肺炎支原体的早期种特异性免疫原性蛋白。利用体外诱变技术将P46基因中三个编码色氨酸的TGA密码子替换为TGG。通过螯合肽标签系统在大肠杆菌中表达突变的P46基因。纯化的重组P46成功用于酶联免疫吸附测定，以检测猪血清中抗猪肺炎支原体的抗体。它与感染絮状支原体、猪鼻支原体或猪滑膜支原体的猪血清无交叉反应。用这种方法，猪感染后2周内即可检测到猪支原体肺炎。
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Recombinant 46-kilodalton surface antigen (P46) of Mycoplasma hyopneumoniae expressed in Escherichia coli can be used for early specific diagnosis of mycoplasmal pneumonia of swine by enzyme-linked immunosorbent assay.在大肠杆菌中表达的猪肺炎支原体重组46千道尔顿表面抗原（P46）可用于通过酶联免疫吸附测定法对猪支原体肺炎进行早期特异性诊断。
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