Nicolet J, Paroz P, Bruggmann S
Res Vet Sci. 1980 Nov;29(3):305-9.
The preparation of an antigen of Mycoplasma hyopneumoniae containing selected proteins after treatment with the neutral detergent Tween 20 and purification on Sephadex G25 is described. The antigenic activity and the specificity were determined by means of an enzyme linked immunosorbent assay and compared with that of a whole-cell, SDS-solubilised antigen. The Tween 20 purified antigen showed a comparable activity but a higher specificity.
描述了猪肺炎支原体抗原的制备方法，该抗原包含经中性去污剂吐温20处理并在葡聚糖凝胶G25上纯化后的选定蛋白质。通过酶联免疫吸附测定法测定抗原活性和特异性，并与全细胞、十二烷基硫酸钠溶解抗原进行比较。吐温20纯化抗原显示出相当的活性，但特异性更高。
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