Schaller K L, Krzemien D M, Yarowsky P J, Krueger B K, Caldwell J H
Department of Cellular and Structural Biology, University of Colorado Health Sciences Center, Denver 80262, USA.
J Neurosci. 1995 May;15(5 Pt 1):3231-42. doi: 10.1523/JNEUROSCI.15-05-03231.1995.
A novel, voltage-gated sodium channel cDNA, designated NaCh6, has been isolated from the rat central and peripheral nervous systems. RNase protection assays showed that NaCh6 is highly expressed in the brain, and NaCh6 mRNA is as abundant or more abundant than the mRNAs for previously identified rat brain sodium channels. In situ hybridization demonstrated that a wide variety of neurons express NaCh6, including motor neurons in the brainstem and spinal cord, cerebellar granule cells, and pyramidal and granule cells of the hippocampus. RT-PCR and/or in situ hybridization showed that astrocytes and Schwann cells express NaCh6. Thus, this sodium channel is broadly distributed throughout the nervous system and is shown to be expressed in both neurons and glial cells.
一种名为NaCh6的新型电压门控钠通道cDNA已从大鼠中枢和外周神经系统中分离出来。核糖核酸酶保护分析表明，NaCh6在脑中高度表达，且NaCh6 mRNA的丰度与先前鉴定的大鼠脑钠通道的mRNA相当或更高。原位杂交显示，多种神经元表达NaCh6，包括脑干和脊髓中的运动神经元、小脑颗粒细胞以及海马体的锥体细胞和颗粒细胞。逆转录聚合酶链反应和/或原位杂交表明，星形胶质细胞和雪旺氏细胞表达NaCh6。因此，这种钠通道广泛分布于整个神经系统，并且在神经元和神经胶质细胞中均有表达。
