Takahashi T, Momiyama A
Department of Neurophysiology, University of Tokyo, Japan.
Jpn J Physiol. 1994;44 Suppl 2:S97-9.
Glycine receptor Cl- channels underlying inhibitory synaptic currents (IPSCs) were characterized with patch clamp methods applied to thin slices of rat spinal cord. The channels had multiple conductance states with their mean open time being similar to the mean decay time constant of IPSCs. During postnatal development, channel open time became gradually shorter, concomitantly with the decay time of IPSCs. Single channel currents of alpha-homomeric glycine receptors expressed in Xenopus oocytes from cRNAs showed that the adult type receptor had much faster kinetics than the fetal one. It is suggested that the molecular switching of glycine receptor alpha subunits accelerate the time course of inhibitory synaptic responses.
通过应用于大鼠脊髓薄片的膜片钳方法对抑制性突触电流（IPSCs）所基于的甘氨酸受体氯离子通道进行了表征。这些通道具有多种电导状态，其平均开放时间与IPSCs的平均衰减时间常数相似。在出生后的发育过程中，通道开放时间逐渐缩短，同时IPSCs的衰减时间也相应缩短。从cRNAs在非洲爪蟾卵母细胞中表达的α-同聚甘氨酸受体的单通道电流表明，成年型受体的动力学比胎儿型受体快得多。这表明甘氨酸受体α亚基的分子转换加速了抑制性突触反应的时间进程。
