Ostlund E, Mendez C F, Jacobsson G, Fryckstedt J, Meister B, Aperia A
Department of Women and Child Health, St. Göran's Children's Hospital, Stockholm, Sweden.
Kidney Int. 1995 Mar;47(3):766-73. doi: 10.1038/ki.1995.117.
PKC plays a central role for the regulation of renal function. PKC consists of a family of isoenzymes. By employing Northern blot techniques we have demonstrated that mRNA transcripts for the classical Ca(2+)-dependent, diacylglycerol-activated isoform alpha, the novel, Ca(2+)-independent isoform delta and the atypical isoform zeta are abundantly expressed in the rat kidney. The novel PKC-epsilon was weakly expressed. The classical PKCs beta I, beta II and gamma could not be detected. The mRNA expression of PKC-delta and -zeta increased with age. The intrarenal localization of PKC-alpha, -delta and -zeta isoforms were studied in the adult kidney using in situ hybridization. In the cortex, the PKC-alpha isoform showed the strongest hybridization signal. PKC alpha, delta and zeta were all distributed in the outer medulla. The PKC-alpha probe detected particularly strong signal in the outer stripe of the outer medulla. Western blot confirmed the presence of the PKC-alpha, -delta and -zeta enzymes in renal tissue. The results show cell-specific and developmentally-dependent expression of three types of PKC isoforms with different responses to diacylglycerol and calcium. The developmental increase of both PKC-delta and PKC-zeta suggests a specific role for these isoforms for the functional regulation of the mature kidney.
蛋白激酶C（PKC）在肾功能调节中起核心作用。PKC由一组同工酶组成。通过Northern印迹技术，我们已证明，经典的钙（Ca2+）依赖性、二酰基甘油激活的α同工型、新型的非钙（Ca2+）依赖性δ同工型以及非典型的ζ同工型的mRNA转录本在大鼠肾脏中大量表达。新型PKC-ε表达较弱。未检测到经典的PKC-βI、βII和γ。PKC-δ和-ζ的mRNA表达随年龄增加。利用原位杂交技术研究了成年肾脏中PKC-α、-δ和-ζ同工型的肾内定位。在皮质中，PKC-α同工型显示出最强的杂交信号。PKC-α、δ和ζ均分布在外髓质。PKC-α探针在外髓质的外带检测到特别强的信号。蛋白质印迹法证实了肾组织中存在PKC-α、-δ和-ζ酶。结果显示了三种类型的PKC同工型的细胞特异性和发育依赖性表达，它们对二酰基甘油和钙有不同反应。PKC-δ和PKC-ζ的发育性增加表明这些同工型在成熟肾脏的功能调节中具有特定作用。
