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[毛细血管显微镜法在测定海洋中微生物总数和生物量方面的应用]

[Application of the method of capillary microscopy to determing the total population and biomass of microorganisms in seas and oceans].

作者信息

Mitskevich I N

出版信息

Mikrobiologiia. 1975 Mar-Apr;44(2):317-20.

PMID:775259
Abstract

The total number and biomass of microorganisms in samples of sea water were determined by direct counting in capillaries by Perfiliev and Gabe after preliminary concentration of water by filtering it through membrane filters. Reliability of the figures obtained upon concentration of diluted bacterial suspensions (10(3) to 10(5) cells/ml) was checked with dilutions of the cell suspension of Bacterium parvulum. The total number of the microorganisms was determined in 22 samples of sea water fixed with 4 to 5 per cent formalin, and deviations for each capillary were calculated.

摘要

在通过膜滤器过滤对海水进行初步浓缩之后,按照佩尔菲利耶夫和加贝的方法,通过在毛细管中直接计数来测定海水样本中微生物的总数和生物量。用细小杆菌细胞悬液的稀释液对稀释后的细菌悬液(10³至10⁵个细胞/毫升)浓缩后所获数据的可靠性进行了检验。在22份用4%至5%福尔马林固定的海水样本中测定了微生物的总数,并计算了每根毛细管的偏差。

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