Yamaguchi M, Oishi K, Isogai M
Laboratory of Metabolism and Endocrinology, Graduate School of Nutritional Sciences, University of Shizuoka, Japan.
Mol Cell Biochem. 1995 Jan 12;142(1):35-41. doi: 10.1007/BF00928911.
The effect of refeeding on the expression of Ca(2+)-binding protein regucalcin mRNA in the liver of fasted rats was investigated. When rats were fasted overnight, the hepatic regucalcin mRNA level was reduced about 70% of that in feeding rats. Refeeding produced a remarkable elevation of hepatic regucalcin mRNA level (about 150-170% of fasted rats). Liver regucalcin concentration was appreciably increased by refeeding, although it was not altered by fasting. The oral administration of glucose (2 g/kg body weight) to fasted rats caused a significant increase in hepatic regucalcin mRNA level. Moreover, hepatic regucalcin mRNA level was clearly elevated by a single subcutaneous administration of insulin (10 and 100 U/kg) to fasted rats. The hormonal effect was not further enhanced by the simultaneous administration of calcium chloride (250 mg Ca/kg) to fasted rats, although calcium administration stimulated regucalcin mRNA expression in the liver. The present study suggests that the expression of hepatic regucalcin mRNA stimulated by refeeding is significantly involved in the action of insulin and/or calcium as stimulating factors.
研究了再喂养对禁食大鼠肝脏中钙结合蛋白调节素mRNA表达的影响。当大鼠禁食过夜时，肝脏调节素mRNA水平降至喂食大鼠的约70%。再喂养使肝脏调节素mRNA水平显著升高（约为禁食大鼠的150 - 170%）。尽管禁食对肝脏调节素浓度无影响，但再喂养使其明显增加。给禁食大鼠口服葡萄糖（2 g/kg体重）可使肝脏调节素mRNA水平显著升高。此外，给禁食大鼠单次皮下注射胰岛素（10和100 U/kg）可明显提高肝脏调节素mRNA水平。尽管给禁食大鼠同时注射氯化钙（250 mg Ca/kg）可刺激肝脏中调节素mRNA表达，但激素作用并未进一步增强。本研究表明，再喂养刺激的肝脏调节素mRNA表达与胰岛素和/或钙作为刺激因子的作用密切相关。
