Bandi C, La Rosa G, Bardin M G, Damiani G, Comincini S, Tasciotti L, Pozio E
Istituto di Patologia Generale Veterinaria, Università di Milano, Italy.
Parasitology. 1995 May;110 ( Pt 4):401-7. doi: 10.1017/s003118200006474x.
Eight taxa have recently been proposed as being encompassed by the genus Trichinella on the basis of allozyme and biological data. In this paper we show that an analogous 8 taxon structure for this genus results from the random amplified polymorphic DNAs (RAPDs). Five 10-mer or 20-mer primers were used under different polymerase chain reaction (PCR) conditions to produce multiband RAPD fingerprints from muscle larvae of 40 isolates of Trichinella spp. The resulting RAPD data were analysed following the numerical taxonomic approach, and the resulting classification was compared to that derived from allozyme data. The agreement found between allozymes and RAPDs, while supporting the polyspecific structure of the genus Trichinella, confirms the potential of RAPDs as a tool for the detection of cryptic species. The selected primers were tested on individual muscle larvae in an attempt to standardize a RAPD assay for the routine identification of the 8 taxa of Trichinella. Only 1 of the 5 primers yielded reproducible fingerprints from the single larvae. Using this primer, the 5 species and the 3 other taxa of the genus Trichinella can be identified in a single assay without the need for massive in vivo parasite production.
最近基于等位酶和生物学数据，有8个分类单元被提议归入旋毛虫属。在本文中，我们表明该属类似的8个分类单元结构源自随机扩增多态性DNA（RAPD）。在不同的聚合酶链反应（PCR）条件下，使用5种10聚体或20聚体引物，从40株旋毛虫属的肌肉幼虫中产生多带RAPD指纹图谱。按照数值分类学方法分析所得的RAPD数据，并将所得分类与源自等位酶数据的分类进行比较。在等位酶和RAPD之间发现的一致性，在支持旋毛虫属多物种结构的同时，证实了RAPD作为检测隐性物种工具的潜力。对所选引物在单个肌肉幼虫上进行测试，试图标准化一种用于常规鉴定旋毛虫属8个分类单元的RAPD分析方法。5种引物中只有1种能从单个幼虫产生可重复的指纹图谱。使用该引物，无需大量体内寄生虫繁殖，在一次分析中就能鉴定旋毛虫属的5个物种和其他3个分类单元。
