Liu M Y, Zhu X P, Xu K C, Lu Q, Boireau P
Quartermaster University of PLA, 175 Xian Road, Changchun 130062, P. R. China.
Parasite. 2001 Jun;8(2 Suppl):S34-8. doi: 10.1051/parasite/200108s2034.
The most recent taxonomic revision in Trichinella genus included 10 taxa. Trichinella spiralis (Ts) is a thoroughly studied species but most of the isolates came from the Europe or American continents. Few information is available from China about the Trichinella isolates and their diversity. In this report two Chinese isolates were characterized and compared with European strains. The in vitro release of newborn larvae (NBL) was determined for the two species. NBL is observed in supernatant of cell culture after adults purification on day 4 post infection (pi) with Ts, and on day 5 pi with Trichinella nativa (Tna). A new parameter was thus proposed to characterize Trichinella strains. Restriction fragment length polymorphism (RFLP) of genomic DNA and random amplified polymorphic cDNA (RAPD) were used to define genetic variability among different isolates. Species specific pattern could be obtained with RAPD but it was far more difficult to get geographical markers for Trichinella using these methods. A low genetic variability in Trichinella species (i.e. a strong "clonality") is suggested.
旋毛虫属最近的分类学修订包括10个分类单元。旋毛形线虫（Ts）是一个经过充分研究的物种，但大多数分离株来自欧洲或美洲大陆。关于中国旋毛虫分离株及其多样性的信息很少。在本报告中，对两个中国分离株进行了特征分析，并与欧洲菌株进行了比较。测定了这两个物种新生幼虫（NBL）的体外释放情况。在用旋毛形线虫感染后第4天（pi）对成虫进行纯化后，在细胞培养上清液中观察到NBL，在用本地毛形线虫（Tna）感染后第5天pi观察到NBL。因此提出了一个新的参数来表征旋毛虫菌株。基因组DNA的限制性片段长度多态性（RFLP）和随机扩增多态性cDNA（RAPD）被用于定义不同分离株之间的遗传变异性。用RAPD可以获得物种特异性模式，但使用这些方法获得旋毛虫的地理标记要困难得多。这表明旋毛虫物种的遗传变异性较低（即强烈的“克隆性”）。
