Hoffman D C, Anderson R C, DuBois M L, Prescott D M
University of Colorado, Department of Molecular, Cellular and Developmental Biology, Boulder 80309-0347, USA.
Nucleic Acids Res. 1995 Apr 25;23(8):1279-83. doi: 10.1093/nar/23.8.1279.
The macronuclear genome of hypotrichous ciliates consists of DNA molecules of gene-sized length. A macronuclear DNA molecule contains a single coding region. We have analyzed the many hypotrich macronuclear DNA sequences sequenced by us and others. No highly conserved promoter sequences nor replication initiation sequences have been identified in the 5' nor in the 3' non-translated regions, suggesting that promoter function in hypotrichs may differ from other eukaryotes. The macronuclear genes are intron-poor; approximately 19% of the genes sequenced to date have one to three introns. Not all macronuclear DNA molecules may be transcribed; some macronuclear molecules may not have any coding function. Codon bias in hypotrichs is different in many respects from other ciliates and from other eukaryotes.
腹毛目纤毛虫的大核基因组由基因大小长度的DNA分子组成。一个大核DNA分子包含一个单一的编码区域。我们分析了由我们自己和其他人测序的许多腹毛目大核DNA序列。在5'和3'非翻译区均未鉴定到高度保守的启动子序列或复制起始序列，这表明腹毛目的启动子功能可能与其他真核生物不同。大核基因内含子较少；迄今为止测序的基因中约19%有一到三个内含子。并非所有大核DNA分子都会被转录；一些大核分子可能没有任何编码功能。腹毛目的密码子偏好性在许多方面与其他纤毛虫和其他真核生物不同。
