Singh V, Chaudhari S S, Kumar S, Chhabra M B
Department of Veterinary Parasitology, Haryana Agricultural University, Hisar, India.
Vet Parasitol. 1995 Feb;56(4):261-7. doi: 10.1016/0304-4017(94)00689-a.
An enzyme-linked immunosorbent assay (ELISA) was employed for the detection of Trypanosoma evansi antigens in serum samples of field cases of buffaloes and horses in northern India. In 323 naturally infected/suspected buffaloes, circulating antigenaemia was detected in 180 (55.72%), whereas parasitaemia by wet blood smear examination was found in 62 (19.19%) only. The antigen-ELISA was positive in 47 of the 62 parasitologically proven cases and in 86 of the 116 cases with anti-trypanosome antibodies detected by ELISA. Of the 80 horses examined antigen-ELISA was positive in 45 (56.75%) sera. The antigen-detection assay was positive in 14 of the 19 parasitaemic cases whereas the antibody-detection assay was positive in 18 of the 30 parasitaemic cases. In the present study, antigen-ELISA was found to be more sensitive and specific compared with antibody-ELISA and wet blood examination, and could prove a useful tool for epidemiological studies of latent trypanosomosis in livestock.
采用酶联免疫吸附测定（ELISA）法检测印度北部水牛和马的野外病例血清样本中的伊氏锥虫抗原。在323头自然感染/疑似感染的水牛中，180头（55.72%）检测到循环抗原血症，而通过湿血涂片检查仅在62头（19.19%）中发现虫血症。在62例经寄生虫学证实的病例中，47例抗原ELISA呈阳性，在116例经ELISA检测出抗锥虫抗体的病例中，86例呈阳性。在检查的80匹马中，45份血清（56.75%）抗原ELISA呈阳性。在19例虫血症病例中，14例抗原检测试验呈阳性，而在30例虫血症病例中，18例抗体检测试验呈阳性。在本研究中，发现抗原ELISA与抗体ELISA和湿血检查相比更敏感、特异，可作为家畜潜在锥虫病流行病学研究的有用工具。
