Pasumarthi K B, Jin Y, Bock M E, Lytras A, Kardami E, Cattini P A
Department of Physiology, University of Manitoba, Winnipeg, Canada.
Ann N Y Acad Sci. 1995 Mar 27;752:406-16. doi: 10.1111/j.1749-6632.1995.tb17448.x.
We used reverse transcriptase-polymerase chain reaction (RT-PCR) to clone fibroblast growth factor receptor (FGFR) 1 isoforms from embryonic mouse heart and as a more sensitive method to characterize FGFR1 RNA expression in embryonic and adult mouse hearts. We describe the cloning of both full-length short (2259 base pairs) and long (2526 base pairs) FGFR1 isoform cDNAs which generated 86 and 102 kilodalton proteins, respectively, following in vitro translation. An assessment of FGFR1 RNA indicates that FGFR1-IIIc is the major form in both the embryonic and adult heart but there is an approximately 8.5-fold decrease in RNA levels in the adult. Differential RNA blotting as well as RT-PCR analyses are consistent with a switch in the relative expression of the short versus long FGFR1 isoforms during heart development. The long isoforms are more abundant in the embryo and the short isoforms predominate in the adult. This may be important in the regulation of growth and development of the heart.
我们使用逆转录聚合酶链反应（RT-PCR）从胚胎小鼠心脏中克隆成纤维细胞生长因子受体（FGFR）1亚型，并将其作为一种更灵敏的方法来表征FGFR1 RNA在胚胎和成年小鼠心脏中的表达。我们描述了全长短（2259个碱基对）和长（2526个碱基对）FGFR1亚型cDNA的克隆，体外翻译后分别产生86和102千道尔顿的蛋白质。对FGFR1 RNA的评估表明，FGFR1-IIIc是胚胎和成年心脏中的主要形式，但成年心脏中RNA水平大约下降了8.5倍。差异RNA印迹以及RT-PCR分析与心脏发育过程中短与长FGFR1亚型相对表达的转变一致。长亚型在胚胎中更为丰富，而短亚型在成年中占主导。这可能对心脏的生长和发育调节很重要。
