Stevens P A, Wissel H, Sieger D, Meienreis-Sudau V, Rüstow B
Department of Neonatology, University Children's Hospital, Humboldt University, Berlin, Germany.
Biochem J. 1995 May 15;308 ( Pt 1)(Pt 1):77-81. doi: 10.1042/bj3080077.
Antibodies against a type II pneumocyte component were developed by an auto-anti-idiotypic approach using surfactant-associated protein A (SP-A) as the immunogen. The antibodies recognize an SP-A-binding protein (approximately 170-200 kDa under non-reducing conditions, 55 kDa under reducing conditions) on the cell membrane of rat type II pneumocytes. One of the antibodies competes with SP-A for binding to type II cells. In immunization assays in vitro, with this antibody as the antigen, anti-SP-A antibodies have been generated. The SP-A-binding cell membrane protein recognized by this auto-anti-idiotypic antibody may be involved in the interaction of extracellular SP-A with the type II pneumocyte and may play a role in the regulation of alveolar surfactant metabolism.
通过以表面活性剂相关蛋白A（SP-A）作为免疫原的自身抗独特型方法，制备了针对II型肺细胞成分的抗体。这些抗体识别大鼠II型肺细胞膜上的一种SP-A结合蛋白（非还原条件下约为170 - 200 kDa，还原条件下为55 kDa）。其中一种抗体与SP-A竞争结合II型细胞。在体外免疫测定中，以该抗体为抗原，已产生了抗SP-A抗体。这种自身抗独特型抗体识别的SP-A结合细胞膜蛋白可能参与细胞外SP-A与II型肺细胞的相互作用，并可能在肺泡表面活性物质代谢的调节中发挥作用。
