Bujía J, Sittinger M
Klinik und Poliklinik für Hals-Nasen-Ohren-Krankheiten, Ludwig-Maximilians-Universität, München.
Laryngorhinootologie. 1995 Mar;74(3):183-7. doi: 10.1055/s-2007-997715.
Within the scope of producing cartilage tissue in a three-dimensional culture design, the stability of the used delivery substance in-vitro tissue product has to be improved. For this, carrier materials consisting of bioresorbable polymers, e. g. poly(L[+]-lactic acid) and poly(glycolic acid) can be used. In respect of the biocompatibility of these polymers, the effect of degradation products on chondrocytes is of major interest. The available biomaterials were tested on chondrocytes in form of their monomers, glycolic acid and L(+)-lactic acid. Effects in regard of cell activity were determined with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide( MTT)test. A non-pH-effect was examined by buffering with concentrated NaOH. In a short-term testing with increasing monomer concentrations as well as in a test over a twelve-day period, L(+)-lactic acid proved to have a lower cytotoxic effect on chondrocytes than glycolic acid. Similar results were obtained with buffered culture media. Therefore, poly(L[+]-lactic acid) can be recommended for the development of chondrocytes-polymer constructs for in-vitro engineering of cartilage tissue.
在三维培养设计中生产软骨组织的范围内，必须提高所用递送物质在体外组织产品中的稳定性。为此，可以使用由生物可吸收聚合物组成的载体材料，例如聚（L[+]-乳酸）和聚乙醇酸。就这些聚合物的生物相容性而言，降解产物对软骨细胞的影响是主要关注点。现有的生物材料以其单体乙醇酸和L（+）-乳酸的形式在软骨细胞上进行了测试。用3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐（MTT）试验测定细胞活性方面的影响。通过用浓氢氧化钠缓冲来检查非pH效应。在单体浓度增加的短期测试以及为期十二天的测试中，L（+）-乳酸对软骨细胞的细胞毒性作用低于乙醇酸。在缓冲培养基中也获得了类似的结果。因此，聚（L[+]-乳酸）可推荐用于软骨组织体外工程的软骨细胞-聚合物构建体的开发。
