Cossart P
Unité des Interactions Bactéries-Cellules, Institut Pasteur, Paris, France.
Curr Opin Cell Biol. 1995 Feb;7(1):94-101. doi: 10.1016/0955-0674(95)80050-6.
Listeria monocytogenes and other bacterial pathogens move in the host cell cytoplasm, propelled by continuous actin assembly at one pole of the bacterium. This actin-based motility requires the presence of the bacterial proteins ActA on L. monocytogenes and IcsA on Shigella flexneri. There have been several major discoveries in the past year: the discovery of the polar distribution of ActA and IcsA on the bacterial surface; the demonstration that bacterial ActA is phosphorylated in infected host cells; the involvement of some host cell proteins, particularly profilin; and the dramatic effect of ActA expression in transfected eukaryotic cells. A cell-free system that reconstitutes faithfully the actin-based motility of L. monocytogenes promises to be instrumental in the further dissection of this fascinating phenomenon.
单核细胞增生李斯特菌和其他细菌病原体在宿主细胞胞质中移动，由细菌一端持续的肌动蛋白组装推动。这种基于肌动蛋白的运动需要单核细胞增生李斯特菌上的细菌蛋白ActA和福氏志贺菌上的IcsA的存在。过去一年有几项重大发现：ActA和IcsA在细菌表面的极性分布的发现；证明细菌ActA在受感染的宿主细胞中被磷酸化；一些宿主细胞蛋白的参与，特别是肌动蛋白单体结合蛋白；以及ActA在转染的真核细胞中的显著作用。一个能如实地重建单核细胞增生李斯特菌基于肌动蛋白的运动的无细胞系统有望有助于进一步剖析这一迷人现象。
