Goldberg M B, Theriot J A, Sansonetti P J
Unite de Pathogenie Microbienne Moleculaire, Institut Pasteur, Paris, France.
Infect Immun. 1994 Dec;62(12):5664-8. doi: 10.1128/iai.62.12.5664-5668.1994.
After lysing the phagocytic vacuole, Shigella spp. accumulate filaments of polymerized actin on their surface at one pole, leading to the formation of actin tails that enable them to move through the cytoplasm. We have recently demonstrated that the Shigella protein IcsA is located at the pole that is adjacent to the growing end of the actin tail (M. B. Goldberg, O. Barzu, C. Parsot, and P. J. Sansonetti, J. Bacteriol. 175:2189-2196, 1993). Not every bacterium that is observed within the cytoplasm has an actin tail. The factors that determine when a bacterium will form a tail are unknown. Here we demonstrate that at the moment of initiation of movement, Shigella spp. are frequently in the process of division. Furthermore, the expression of IcsA on the surface of the bacteria occurs in a growth phase-dependent fashion, suggesting that the surface expression of IcsA per se determines the observed association of bacterial division with movement.
在裂解吞噬泡后，志贺氏菌属在其表面的一极积累聚合肌动蛋白丝，导致形成肌动蛋白尾，使其能够在细胞质中移动。我们最近证明，志贺氏菌蛋白IcsA位于与肌动蛋白尾生长末端相邻的极（M.B.戈德堡、O.巴尔祖、C.帕尔索和P.J.桑索尼蒂，《细菌学杂志》175:2189 - 2196，1993年）。并非在细胞质中观察到的每个细菌都有肌动蛋白尾。决定细菌何时形成尾的因素尚不清楚。在这里我们证明，在运动开始时，志贺氏菌属经常处于分裂过程中。此外，IcsA在细菌表面的表达以生长阶段依赖的方式发生，这表明IcsA本身的表面表达决定了观察到的细菌分裂与运动的关联。
