Tebbe B, Mazur L, Stadler R, Orfanos C E
Department of Dermatology, University Medical Center Benjamin Franklin, Free University of Berlin, Germany.
Br J Dermatol. 1995 Jan;132(1):25-31. doi: 10.1111/j.1365-2133.1995.tb08620.x.
An immunohistochemical analysis of skin biopsies was performed in 18 patients with cutaneous lupus erythematosus (LE), using the alkaline phosphatase and monoclonal anti-alkaline phosphatase method (APAAP). The study group was subdivided on the basis of clinical criteria into 10 patients with chronic discoid LE (CDLE) and eight patients with subacute cutaneous LE (SCLE). Using a panel of monoclonal antibodies the following results were obtained: (i) ICAM-1 was expressed on epidermal keratinocytes, dermal inflammatory cells, and endothelial cells in most biopsies, whereas LFA-1 was confined to the dermis. Attachments between keratinocytes or endothelial cells and activated T lymphocytes via ICAM-1/LFA-1 may be a possible mechanism of target/effector recognition in cutaneous LE. (ii) HLA-DR was expressed on epidermal keratinocytes and cells of the dermal infiltrate, but not on endothelial cells. HLA-DR+ cells probably function as antigen-presenting cells, leading to major histocompatibility complex-restricted cellular cytotoxicity in cutaneous LE. (iii) Interleukin 2 receptor expression on dermal inflammatory cells was weak, indicating non-specific activation of T lymphocytes. (iv) The dermal inflammatory cells were T lymphocytes, mainly of the helper/inducer subtype. B lymphocytes were rarely found in the dermis. In general, no significant immunohistochemical differences were found between CDLE and SCLE, suggesting that these variants represent clinical subtypes rather than different pathogenetic entities.
采用碱性磷酸酶和单克隆抗碱性磷酸酶方法（APAAP），对18例皮肤红斑狼疮（LE）患者的皮肤活检标本进行了免疫组织化学分析。根据临床标准，将研究组分为10例慢性盘状LE（CDLE）患者和8例亚急性皮肤LE（SCLE）患者。使用一组单克隆抗体获得了以下结果：（i）在大多数活检标本中，ICAM - 1在表皮角质形成细胞、真皮炎性细胞和内皮细胞上表达，而LFA - 1局限于真皮。角质形成细胞或内皮细胞与活化T淋巴细胞通过ICAM - 1/LFA - 1的附着可能是皮肤LE中靶细胞/效应细胞识别的一种可能机制。（ii）HLA - DR在表皮角质形成细胞和真皮浸润细胞上表达，但在内皮细胞上不表达。HLA - DR +细胞可能作为抗原呈递细胞，导致皮肤LE中主要组织相容性复合体限制的细胞毒性。（iii）真皮炎性细胞上白细胞介素2受体表达较弱，表明T淋巴细胞的非特异性活化。（iv）真皮炎性细胞是T淋巴细胞，主要是辅助/诱导亚型。在真皮中很少发现B淋巴细胞。一般来说，CDLE和SCLE之间未发现明显的免疫组织化学差异,这表明这些变体代表临床亚型而非不同的致病实体。
