Stein L F, Saed G M, Fivenson D P
Department of Dermatology, Henry Ford Hospital, Detroit, MI 48202, USA.
J Am Acad Dermatol. 1997 Feb;36(2 Pt 1):191-6. doi: 10.1016/s0190-9622(97)70279-2.
A variety of immunologic abnormalities have been described in systemic and experimental lupus erythematosus (LE). Several T-cell defects, especially in helper T (Th) cell cytokines, have been reported.
Our purpose was to identify the Th cytokine profile in cutaneous LE.
Total RNA was extracted from punch biopsy specimens from 19 patients with cutaneous LE (nine, discoid LE; two, subacute cutaneous LE; and eight, systemic LE) and from four healthy control subjects. RNA was reverse transcribed into complementary DNA and amplified with polymerase chain reaction (PCR) primers specific for interleukin-2 (IL-2), IL-4, IL-5, IL-10, interferon gamma (IFN-gamma), and beta actin. PCR products were detected by agarose gel electrophoresis and Southern blot with 32P-labeled, nested probes.
Sixteen of 19 cutaneous LE specimens lacked IL-2, all were negative for IL-4, and 10 of 19 had detectable IL-10, whereas IFN-gamma and IL-5 messenger RNAs were present in the majority of LE specimens. IFN-gamma and IL-10 mRNAs were found in all normal skin controls, whereas IL-2, IL-4, and IL-5 mRNAs were undetectable. Functional IFN-gamma protein was evidenced by intercellular adhesion molecule-1 and HLA-DR staining of keratinocytes in nine of nine LE specimens but not in normal skin. The pattern of cytokine mRNAs, intercellular adhesion molecule-1, and/or HLA-DR expression in cutaneous LE specimens did not vary with different subtypes of LE, antinuclear antibody titer, or the magnitude of inflammation.
The presence of IL-5 mRNA in cutaneous LE specimens suggests that Th type 2 cells combine with local IFN-gamma production to augment disease and may be related to the pathophysiology of cutaneous LE.
在系统性红斑狼疮（LE）和实验性红斑狼疮中已描述了多种免疫异常。已报道了几种T细胞缺陷，尤其是辅助性T（Th）细胞细胞因子方面的缺陷。
我们的目的是确定皮肤型LE中的Th细胞因子谱。
从19例皮肤型LE患者（9例盘状LE、2例亚急性皮肤型LE和8例系统性LE）的打孔活检标本以及4名健康对照者中提取总RNA。RNA被逆转录为互补DNA，并用针对白细胞介素-2（IL-2）、IL-4、IL-5、IL-10、干扰素γ（IFN-γ）和β肌动蛋白的聚合酶链反应（PCR）引物进行扩增。PCR产物通过琼脂糖凝胶电泳和用32P标记的巢式探针进行Southern印迹检测。
19例皮肤型LE标本中有16例缺乏IL-2，所有标本IL-4均为阴性，19例中有10例可检测到IL-10，而大多数LE标本中存在IFN-γ和IL-5信使RNA。在所有正常皮肤对照中均发现了IFN-γ和IL-10信使RNA，而未检测到IL-2、IL-4和IL-5信使RNA。在9例LE标本中的9例角质形成细胞的细胞间黏附分子-1和HLA-DR染色显示有功能性IFN-γ蛋白，而正常皮肤中未显示。皮肤型LE标本中细胞因子信使RNA、细胞间黏附分子-1和/或HLA-DR表达模式在不同亚型的LE、抗核抗体滴度或炎症程度方面无差异。
皮肤型LE标本中存在IL-5信使RNA提示2型Th细胞与局部IFN-γ产生相结合以加重疾病，可能与皮肤型LE的病理生理学相关。
