[Detection of polynucleotide fragments using hybridization with probes labelled with digoxigenin].
作者信息
Cîrciumaru R, Anton G, Stoian M, Repanovici R
机构信息
Institut de Virologie Stefan S. Nicolau, Bucarest, Roumanie.
出版信息
Rev Roum Virol. 1994 Jan-Jun;45(1-2):11-8.
PMID:7756159
Abstract
A new nonradioactive system for labeling molecular probes, using digoxygenine, was studied. Detection of ADN-ADN type hybrids formed on a nylon membrane was realised photochemically or by ELISA, dot-blot or Southern-blot. The high sensitivity and specificity of this new system allow to use it for detection of amounts of ADN as small as 5 ng.
摘要
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