Conrads G, Mutters R, Seyfarth I, Pelz K
Clinic of Conservative & Preventive Dentstry and Periodontology, University Hospital (RWTH) Aachen, Germany.
Mol Cell Probes. 1997 Oct;11(5):323-8. doi: 10.1006/mcpr.1997.0124.
We designed oligonucleotides to differentiate between the seven currently known Capnocytophaga species. The oligonucleotides were labelled non-radioactively at the 3' end with digoxigenin. The specificity could be demonstrated in a dot-blot hybridization assay by using the type strains, reference strains, and 37 clinical Capnocytophaga isolates as well as 11 representative strains of other taxa as a template. The sensitivity of the assay was calculated with 10(3) bacteria per dot.
我们设计了寡核苷酸，以区分目前已知的七种二氧化碳嗜纤维菌属菌种。这些寡核苷酸在3'端用洋地黄毒苷进行非放射性标记。通过使用模式菌株、参考菌株、37株临床分离的二氧化碳嗜纤维菌以及11株其他类群的代表性菌株作为模板，在斑点杂交试验中可证明其特异性。该试验的灵敏度以每个斑点10³个细菌进行计算。
