Adachi K, Tateishi M, Horii Y, Nagatomo H, Shimizu T, Makimura S
Department of Veterinary Internal Medicine, Faculty of Agriculture, Miyazaki University, Japan.
J Vet Med Sci. 1995 Feb;57(1):121-3. doi: 10.1292/jvms.57.121.
Serum anti-erythrocyte membrane (RBCm) antibodies and anti-RBCm antibodies eluted from erythrocytes of Babesia gibsoni-infected dogs were examined for immunologic characteristics by immunoblotting analysis. Serum anti-RBCm antibodies of the dogs before and after the experimental inoculation with parasitized erythrocytes reacted with cytoskeletal (bands 1, 2, 2.1, 4.1, 4.2 and 5) and transmembrane structural (band 3) proteins of either self or non-self RBCm. Anti-RBCm antibodies eluted from erythrocytes of B. gibsoni-infected dogs reacted with bands 3 and 4.2 of self and non-self RBCm.
通过免疫印迹分析，检测了吉氏巴贝斯虫感染犬的血清抗红细胞膜（RBCm）抗体以及从感染犬红细胞中洗脱的抗RBCm抗体的免疫学特性。实验接种寄生红细胞前后犬的血清抗RBCm抗体与自身或非自身RBCm的细胞骨架蛋白（条带1、2、2.1、4.1、4.2和5）及跨膜结构蛋白（条带3）发生反应。从吉氏巴贝斯虫感染犬红细胞中洗脱的抗RBCm抗体与自身和非自身RBCm的条带3和4.2发生反应。
